Cyclosporin A (CyA) was assayed by high performance liquid chromatography (HPLC) following extraction of the whole blood or plasma with diethyl ether and chromatography on a Sep-Pak Silica cartridge. Using carefully chosen volumes and ratios of ethyl acetate and hexane in the eluting sequences on the Sep-Pak, the major interferent present in normal blood and serum was eliminated.
References
1.
CalneRYRollesITWhiteDJGCyclosporin A initially as the only immunosuppressant in 34 recipients of cadaveric organs: 32 kidneys. 2 pancreases and 2 livers. Lancet1980; ii: 1033–6.
2.
KeownPAStillerCRVlanRAImmunological and pharmacological monitoring in the clinical use of Cyclosporin-A. Lancet1981; i: 686–9.
3.
Leyland-JonesBClarkAKreisWHigh pressure liquid chromatographic determination of Cyclosporin-A in human plasma. Rex Comtnun Chem Pathol Pharmacol1982; 37: 431–44.
4.
TaubeDHWilliamsD GwynHarleyBDifferentiation between allograft rejection and cyclosporin nephrotoxicity in renal-transplant recipients. Lancet1985; i: 171–4.
5.
VargheseZChanMKSteeleLVHow to measure cyclosporin. Lancet1984; 1: 1407–8.
6.
OosthuizenMMJJacobsDRMyburghJ A.Preparation, extraction and high performance liquid chromatography of cyclosporin A from plasma that contains interfering compounds. Ann Clin Biochem1985; 22: 402–7.
7.
CarrulhcrsSGFreemanDJKoeglcrJCSimplified liquid chromatographic analysis for Cyclosporin-A and comparison with radioimmunoassay. Clin Chem1983; 29: 180–3.
8.
AllwoodMCLawranccR. High pressure liquid chromatographic determination of cyclosporin A in plasma. J Clin Hosp Pharm1981; 6: 195–9.
9.
BowersLDMalhewsS E. Investigation of the mechanism of peak broadening observed in the high-performance liquid chromatographic analysis of cyclosporine. J Chromatog1985; 333: 231–8.
10.
KabraPMWallJHBlanckaertN.Solid-phase extraction and liquid chromatrography for improved assay of cyclosporine in whole blood or plasma. Clin Chem1985; 31: 1717–20.
