A method is described for the measurement of acetate in plasma by an enzymatic technique using acetate kinase, either manually or with the Multistat centrifugal analyser. The technique was used to compare concentrations in diabetic and non-diabetic subjects and to determine correlates with other intermediary metabolites.
References
1.
RemeseyCDemigneC. Determination of volatile fatty acids in plasma after ethanolic extraction. Biochem J1974; 141: 85–91.
2.
BuckleyBMWilliamsonDH. Origins of blood acetate in the rat. Biochem J1977; 166: 539–45.
3.
KnowlesSEJarrettJGFilsellOHBallardFJ. Production and utilisation of acetate in mammals. Biochem J1974; 142: 401–11.
4.
SeufertCDGrafMJansonGKuhnASolingHD. Formation of free acetate by isolated perfused livers from normal, starved and diabetic rats. Biochem Biophys Res Commun1974; 57: 901–9.
5.
MurthyVKSteinerG. Hepatic acetic thiokinase: Possible regulatory step in lipogenesis. Metab Clin Exp.1972; 21: 213–21.
6.
KrebsHAJohnsonWA. Metabolism of ketonic acids in animal tissues. Biochem J1937; 31: 645–60.
7.
LakerMFMansellMA. Measurement of acetate in aqueous solutions and plasma by gas phase chromatography using a porous polymer stationary phase. Ann Clin BioChem.1978; 15: 228–32.
8.
BergmeyerHUMolleringH. Acetate. In: BergmeyerHU, ed. Methods of Enzymatic Analysis. 2nd edn, Vol. 3. New York: Academic Press, 1974; 1520.
9.
TrivinCLenoirFBretaudiereJPSachsC. Enzymatic determination of acetate in serum or plasma using a centrifugal fast analyser. Clin Chim Acta1982; 121: 43–50.
10.
AlbertiKGMMRecordCOWilliamsonDHMetabolic changes in active chronic hepatitis. Clin Sci.1972; 42: 591–605.
MorganCRLazarowA. Immunoassay of insulin: Two antibody system. Plasma insulin levels in normal, sub-diabetic, and diabetic rats. Diabetes1963; 12: 115–26.
13.
BergmeyerHUBerntESchmidtFStorkH. Glucose. In: BergmeyerHU, ed. Methods of Enzymatic Analysis, 2nd edn, Vol. 3. New York: Academic Press, 1974; 1196.
14.
PortFKEasterlingRE. Evaluation of acetate tolerance during highly efficient haemodialysis. Proceedings of the Dialysis Transplant Forum1975; 5: 128–30.