A method is described for measuring the percentage of free testosterone in serum using steady-state gel filtration on micro-columns of Sephadex G50 (fine). The method has a coefficient of variation of 4·0% and up to 20 samples can be processed in a day.
Reference ranges have been established for normal women at different stages of their menstrual cycles and for normal men; there is good agreement with values published by workers using other methods. This method combined with a specific and precise method for measuring total testosterone opens up the possibility for the routine measurement of free testosterone concentration in serum.
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