An accelerated double antibody method has been developed for the radioimmunoassay of pregnancy-associated plasma protein A (PAPP-A) in serum. The workable range for the assay was 0·04–1·8 mg/l of serum. PAPP-A levels were determined in single serum samples from 110 women with prospective normal pregnancies of between 7 and 14 weeks' gestation. The level of pregnancy specific β1 glycoprotein (SP1) was also measured in these samples and normal ranges for PAPP-A and SP1 were constructed from the results obtained.
References
1.
LinTMHalbertSPKieferDCharacterization of four human pregnancy-associated plasma proteins. Am J Obstet Gynecol1974; 118: 223–36.
2.
LinTMHalbertSPKieferDThree pregnancy-associated human plasma proteins. Purification, monospecific antisera and immunological identification. Int Archs Allergy Appl Immun1974; 47: 35–53.
3.
LinTMHalbertSP.Placental localisation of human pregnancy associated plasma proteins. Science1976; 193: 1249–52.
4.
McIntyreJAHsiBFaulkW PageImmunological studies of the human placenta: functional and morphological analysis of pregnancy associated plasma protein A (PAPP-A). Immunology1981; 44: 577–83.
5.
WahlstromTTeisnerBFolkersenJ.Tissue localisation of pregnancy-associated plasma protein A (PAPP-A) in normal placenta. Placenta1981; 2: 253–8.
6.
LinTMHalbertSPSpellacyWN.Relation of obstetric parameters to the concentrations of four pregnancy-associated plasma protein at term in normal gestation. Am J Obstet Gynecol1976; 125: 17–24.
7.
HughesGBischofPWilsonGTests of fetal wellbeing in the third trimester of pregnancy. Brit J Obstet Gynaec1980; 87: 650–6.
8.
SmithRBischofPHughesG. (1981): Studies on pregnancy-associated plasma protein A in the third trimester of pregnancy. Br J Obstet Gynaecol1979; 86: 882–7.
9.
BischofPHaenggeliLSizonenkoMTA radioimmunoassay for the measurement of pregnancy-associated plasma protien A (PAPP-A) in humans. Biol Reprod1981; 24: 1076–81.
10.
FolkersenJGrudzinskasJGHindersonPPregnancy-associated plasma protein A: Circulating levels during normal pregnancy. Am J Obstet Gynecol1981; 139: 910–14.
11.
GordonYBGrudzinskasJGJeffreyDConcentrations of pregnancy-specific-β1-glycoprotein in maternal blood in normal pregnancy and in intrauterine growth retardation. Lancet1977; 1: 331–3.
12.
AnthonyFMassonGMWoodPJ.The radioimmunoassay of a pregnancy-specific-β1-glycoprotein in plasma as a pregnancy test for subfertile women. Br J Obstet Gynaecol1980; 87: 496–500.
13.
GreenwoodFCHunterWMGloverJS.The preparation of 131I-labelled human growth hormone of high specific activity. Biochem J1963; 89: 114–23.
14.
EkinsRP.Basic principles and theory. Br Med Bull1974; 30: 3–11.
15.
SutcliffeRGKukulska-LanglandsCogginsJRStudies on human pregnancy-associated plasma protein A. Biochem J1980; 191: 799–809.
16.
SinosichMJTeisnerBFolkersenJRadioimmunoassay for pregnancy-associated plasma protein A. Clin Chem1982; 28/1, 50–3.
17.
LentonEAGrudzinskasJGGordonYBPregnancy specific β1 glycoprotein and chorionic gonadotrophin in early human pregnancy. Acta Obstet Gynaecol Scand1981; 60: 489–92.
18.
SinosichMJTeisnerBDaveyMPregnancy-associated plasma protein A: Interaction with heparin in crossed affinity immunoelectrophoresis. Aust NZ J Med.1981; 11: 429–33.
19.
BischofPDubergSHerrmannWPregnancy-associated plasma protein A (PAPP-A) and hCG in early pregnancy. Br J Obstet Gynaecol1981; 88: 973–5.
