Influence of Pyridoxal-5′-Phosphate on Temperature Relationship of Aspartate Aminotransferase and Alanine Aminotransferase

Abstract

The influence of pyridoxal-5′-phosphate on the temperature relationship of aspartate aminotransferase and alanine aminotransferase has been determined. On varying the reaction temperature from 25° to 45° both enzymes showed small but significant deviations from a linear Arrhenius relationship. Supplementation with pyridoxal-5′-phosphate in general enhanced the catalytic activity to the same degree over the entire temperature range. Our results corroborate the findings of Rej but not those of Jung et al.