An automated method is described for the determination of chloride in biological fluids using a mercuric 2,4,6-tri-(2-pyridyl)-1,3,5-triazine reagent. Results obtained by this method compared favourably with those obtained by coulometric titration. The same reagent was used manually to measure the small amounts of chloride in sweat. The results obtained agreed well with the direct measurement of chloride using an ion-specific electrode.
References
1.
ZallDMFisherDGarnerMQ. Photometric determination of chlorides in water. Analy Chem1956; 28: 1665–8.
FriedRHoeflmayrJVelosyG. A new highly sensitive method for the determination of chloride in body fluids without protein precipitation. Z. Klin Chem Klin Biochem1972; 10: 280.
4.
FeldkampCSPalmerDJSalancyJAInterference by other halides in the automation of chloride; contributions to the general methodology of continuous flow systems. Z Klin Chem Klin Biochem1974; 4: 146–50.
5.
De JongEBMGoldschmidtHMJVan AlphenACCMAn improved automated method for serum chloride. Clin Chem1980; 26: 1233–4.
6.
GibsonLECookeRE. Test for concentration of electrolytes in sweat in cystic fibrosis of pancreas utilizing pilocarpine by iontophoresis. Paediatrics1959; 23: 545–9.
7.
BroughtonPMGButtolphMAGowenlockAHRecommended scheme for the evaluation of instruments for automatic analysis in the clinical biochemistry laboratory. J Clin Path1969; 22: 278–84.
8.
DriscollJLMartinHF. Detection of brominism by an automated chloride method. Clin Chem1966; 12: 314–8.