The detection limits for protein, when stained with nigrosine after electrophoresis on cellulose acetate, were studied and shown to be: albumin, 3·0 ng; transferrin, 0·8 ng; and Bence-Jones protein, 0·8 ng. This was less than one-hundredth of the previously reported levels. Immuno-fixation, although useful for identification, did not lower the detection limit for Bence-Jones protein.
References
1.
SherwinMRMooreGH. Microzone electrophoresis of unconcentrated cerebrospinal fluid using cellulose acetate strips and nigrosine dye. Am J Clin Path1971; 55: 705–12.
