Immunoelectrophoresis (Laurell-rocket) in ultrathinfilm gels has been applied to the determination of human serum albumin in a model study. Agarose gels of 0·24 mm thickness were used and optimum electrophoretic conditions were determined. Precision was assessed and comparison with conventional immunoelectrophoresis and a chemical method was made. Though precision was poor (CV 4–6%) improvements in dispensing small volumes (0·2 μl) should change this. Good correlation between methods (correlation coefficient 0·89–0·97) was obtained. The advantages of the technique are speed of analysis and low reagent costs.
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