We report a quick method for the detection of cocaine and its major metabolites by thin-layer chromatography with confirmation by gas-liquid chromatography. Because it is possible to use the same chloroform-isopropanol extracts of urine in both procedures, the method is convenient for screening large numbers of urine samples.
References
1.
KaisthaKK.Guide to urine testing in drug abuse prevention and multimodality treatment programs. J Chromatogr1977; 141: 145–96.
2.
WoodsLAMcMahonFGSeeversMH.The estimation of amines in biological material with initial data for cocaine and mescaline. J Pharmacol Exp Ther1951; 101: 188–91.
3.
JavaidJIDekirmenjianEGBrunngraberEGDavisJM.Quantitative determination of cocaine and its metabolites benzoyl ecgonine and ecgonine by gas-liquid chromatography. J Chromatogr1975; 10: 141–9.
4.
KoontzSBesemerDMackeyNPhillipsR.Detection of benzoyl ecgonine (cocaine metabolite) in urine by gas chromatography. J Chromatogr1973; 85: 75–9.
5.
DvorchikHMillerSHGrahamWP.Gas chromatographic determination of cocaine in whole blood and plasma using a nitrogen-sensitive flame ionization detector. J. Chromatogr1977; 135: 141–8.
6.
KaulBMillianSJDavidowB.The development of a radioimmunoassay for detection of cocaine metabolites. J Pharmacol Exp Ther1976; 199: 171–8.
7.
RubinsteinKESchneiderRSUllmanEF.Homogeneous enzyme immunoassay, a new immunochemical technique. Biochim Biophys Res Commun1972; 47: 846–7.
