LDH activity in gastric juice was high in all cases of carcinoma of the stomach investigated provided that the pH of the resting gastric juice was above 5·0. No LDH activity could be detected in gastric aspirate, whether from patients with carcinoma of the stomach or not, when the pH of the resting juice was below 5·0. LDH activity in gastric juice is rapidly and irreversibly inactivated by hydrochloric acid at a pH of 4·5 to 5·0.
References
1.
FigusAISimonL.Screening for gastric carcinoma by determination of lactic acid dehydrogenase activity in the gastric juice. Digestion1972; 7: 266–76.
2.
FaulkWPRiderJASwaderJI.Lactate dehydrogenase in gastric juice: Diagnostic adjunct in human stomach cancer. The Lancet1972; 2: 1115–7.
3.
PiperDWMacounMLBroderickFLFentonBHBuilderJE.The diagnosis of gastric carcinoma by the estimation of enzyme activity in gastric juice. Gastroenterology1963; 45: 614–9.
4.
SimonLFigusAI.Diagnostic value of determination of lactate dehydrogenase and β glucuronidase activity of gastric juice: correlation with a histologic study of the gastric mucosa. Digestion1972; 7: 174–82.
5.
WoollamsRBarrattPJOrwellRLPiperDW.LDH isoenzyme pattern of uninvolved gastric mucosa of patients with gastric carcinoma and benign gastric disease. Digestion1976; 14: 20–8.
6.
Carda-AbellPPerez-CuadradoSMate-JimenezJ.LDH isoenzyme patterns in human gastric mucosa with precancerous changes. Cancer1978; 42: 490–4.
7.
MateJPajaresJMMoreno-OteroRPerez-CuadradoS.Abnormal lactic dehydrogenase patterns in carcinoma of stomach and in peritumoral tissue. Digestion1978; 17: 377–82.
8.
EllisGGoldbergDM.Serum hydroxybutyrate dehydrogenase activity. An improved method. Am J Clin Pathol1971; 56: 627–35.
9.
PrestonJABriereROBatsakisJG.Rapid electrophoretic separation of lactate dehydrogenase isoenzymes on cellulose acetate. Am J Clin Pathol1965; 43: 256–60.
10.
HenryRJChiamoriNGolubOJBerkmanS.Revised spectrophotometric methods for the determination of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase and lactic acid dehydrogenase. Am J Clin Pathol1960; 34: 381–98.
11.
ClelandWW.Dithiothreital, a new protective reagent for SH-groups. Biochemistry1964; 3: 480–2.
12.
RuddellWSJBondESHillMJWaltersCL.Pathogenesis of gastric cancer in pernicious anaemia. The Lancet1978; 1: 521–3.
13.
MacounMLJanetteBPiperDW.Lactic dehydrogenase activity of gastric juice of normal subjects and of patients with gastric ulcer, duodenal ulcer, gastric carcinoma and pernicious anaemia: A preliminary report. Aust Ann Med.1963; 12: 160–5.
14.
PiperDWGriffithEMIrvingLGFentonBH.Value of β glucuronidase activity in gastric juice in the diagnosis of gastric carcinoma. Gastroenterology1966; 51: 172–9.
