A new method is demonstrated for the separation of free and bound fractions in a wide range of immunoassays without the need for centrifugation and decantation. The method employs a novel separator device which, in conjunction with any choice of separating reagent, allows all manipulations to be performed in a single tube simply, rapidly, and safely. The system has potential applications in other fields involving mass transport through selective barriers.
References
1.
CaisMShimoniM.A feasible solvent separation system for immunoassays. Ann Clin Biochem1981; 18: 317–23.
RatcliffeJG.Separation techniques in saturation analysis. Br Med Bull1974; 30: 32–7.
4.
OdellWDSilverCGroverPK.Competitive protein binding assays. Methods of separation of bound from free. In: CameronEHDHillierSGGriffithsK, eds. Steroid immunoassays1975; 207–22. Cardiff: Alpha Omega.
5.
CollinsWPBarnardGJRHennamJF.Factors affecting the choice of separation technique, In: CameronEHDHillierSGGriffithsK, eds. Steroid immunoassays1975; 223–8. Cardiff: Alpha Omega.
6.
ThorellJILarsonSM.Separation technique. In: Radioimmunoassays and related techniques1978; 50–8. Saint Louis. CV Mosby Co.
