Feasibility is demonstrated for a new solvent extraction method to separate free and bound fractions in hapten immunoassays, without the need to centrifuge. A novel mixer-separator device used in conjunction with solvent extraction makes the new separation method simple and rapid, avoids the need for decantation, and has applications for all mass transfer operations involving solvent extraction.
CaisMSlovinESnarskyL.Metalloimmunoassay II. Iron-metallohaptens from estrogen steroids. J Organomeal Chem1978; 160: 223–30.
3.
CaisM.Tests immunologiques a l'aide des complexes organometalliques. Un nouveau concept. L'actualité chimique1979; 7: 14–26.
4.
CaisMTiroshN.Metalloimmunoassay IV. Manganese-labelled metallohaptens via cymantrene derivatives. Bull Soc Chim Belg1981; 90: 27–35.
5.
GandolfiOCaisMDolcettiGMetalloimmunoassay V. Steroid-platinum (II)—O—catecholato complexes: A novel set of metallohaptens. Inorg Chim Acta-Bioinorg Chem in press.
RatcliffeJ G.Separation techniques in saturation analysis. Br Med Bull1974; 30: 32–7.
8.
OdellWDSilverCGroverPK.Competitive protein binding assays. Methods of separation of bound from free. In: CameronEHDHillierSGGriffithsK, eds. Steroid immunoassays1975; 207–22. Cardiff: Alpha Omega.
9.
ThorellJILarsonSM.Separation techniques. In: Radioimmunoassays and related techniques1978; 50–8. Saint Louis: CV Mosby Co.
JowettTPSlaterJDHPiyasenaRDRadioimmunoassay of aldosterone in plasma and urine: validation of a novel separation technique and a rapid urine assay. Clin Sci.1973; 45: 607–23.
12.
CaisMShimoniM.A novel system for mass transport through selective barriers in non-centrifugation immunoassays. Ann Clin Biochem, 1981; 18: 324–9.
