Abstract
An enzyme immunoassay kit for carcinoembryonic antigen (CEA) that uses plastic beads coated with guinea-pig anti-CEA as first antibody and goat anti-CEA conjugated with horseradish peroxidase as second antibody has been evaluated. The method does not involve perchloric acid extraction and therefore avoids a dialysis procedure. It is accurate, sensitive, and inexpensive to operate, and provides values comparable to those obtained with the Roche-CEA Z-Gel radioimmunoassay.
References
1.
Gold
P
Freedman
SO.
Demonstration of tumor-specific antigens in human colonic carcinomata by immunological tolerance and absorption techniques . J Exp Med.
1965 ; 121 : 439 –62 .
2.
Gold
P
Freedman
SO.
Specific carcinoembryonic antigen of the human digestive system . J Exp Med.
1965 ; 122 : 467 –81 .
3.
Reynoso
G
Chu
TM
Holyoke
D
Murphy
GP.
Carcinoembryonic antigen in patients with different cancers . Clin Res.
1971 ; 19 : 672 .
4.
LoGerfo
P
Krupey
J
Hansen
NJ.
Demonstration of an antigen common to several varieties of neoplasia assay using zyrconyl phosphate gel . N Eng J Med.
1971 ; 285 : 138 –41 .
5.
Chu
TM
Reynoso
G.
Evaluation of a new radioimmunoassay method for CEA in plasma with use of zyrconyl phosphate gel . Clin Chem
1972 ; 18 : 918 –22 .
6.
Ashman
LK
Ludbrook
J
Marshall
VR.
Comparison of whole plasma and perchloric acid extracted plasma assays for CEA . Clin Chem Acta
1977 ; 74 : 77 –84 .
7.
Kupchik
HZ
Lowenstein
MS
Feil
M
Rittgers
RA
Zamchek
N.
The disparity of indirect and direct Z-ge assays for CEA . Cancer
1978 ; 42 : 1589 –94 .
8.
Ladenson
JH
Bell
CE.
pH measurement problems affecting assay of CEA . Clin Chem
1975 ; 21 : 255 –7 .
9.
Lowenstein
MS
Kupchik
HZ
Zamcheck
N.
Disparity between CEA-Roche “indirect” and “direct” CEA values: Clinical relevance . New Engl J Med.
1976 ; 294 : 1123 .
10.
Haagensen
DE
Easterdat
RL
Stolle
CA
Wells
SA.
Buffer exchange column for rapid separation of CEA from perchloric acid . Clin Chem
1978 ; 24 : 135 –7 .
11.
Dupchick
NZ
Saravis
CA
Feil
MC
Zamcheck
N.
A rapid dialysis cell in the Z-gel assay for CEA in human serum . J Natl Cancer Inst
1974 ; 52 : 1459 –62 .
12.
Kumar
MS
Carl
KJ
Deodhar
SD.
Improved indirect and direct radioimmunoassay for CEA . Clin Chem
1979 ; 25 : 314 –8 .
13.
McPherson
TA
Koch
M
Coates
JE.
Evaluation of a direct radioimmunoassay for CEA . Clin Biochem
1978 ; 11 : 169 –71 .
14.
Fabricatorian
D
Gallagher
ND.
Two kit assays of carcinoembryonic antigen compared . Clin Chem
1979 ; 25 : 1189 .