The major neutral urinary steroids have been quantitated by capillary gas chromatography and the reproducibility and accuracy of the method have been fully evaluated. Typical intra- and inter-assay coefficients of variation for individual steroids ranged from 4·9 to 10·1% and from 15·6 to 21·5%, respectively. Comparison of steroid values with alternate and commonly employed procedures for measuring urinary 17-oxosteroids, total 17-oxogenic steroids, and cortisol and plasma dehydroepiandrosterone-sulphate yielded correlation coefficients between 0·71 and 0·79. A definite relationship between serum cortisol and urinary trihydroxy-pregnanedione was also shown. Urinary androstenedione metabolites, however, could not be significantly (P>0·05) correlated with the normalised androgen ratio in plasma.
References
1.
GrayCHBrooksRVBaronDNJamesVHT. A critical appraisal of a method estimating urinary 17-oxosteroids and total 17-oxogenic steroids. Lancet1969; 1: 124–7.
2.
MatthijssenCGoldzieherJWFrancoCWrightEL. Analysis of total, grouped, and individual 17-ketosteroids: A critical investigation. Acta Endocrinol (Kbb)1971; 68: 311–33.
3.
MaroulisGBManlimosFSAbrahamGE. Comparison between urinary 17-ketosteroids and serum androgens in hirsute patients. Obstet Gynecol1977; 49: 454–8.
4.
JuddHLMcPhersonRARakoffJSYenSSC. Correlation of the effects of dexamethasone administration on urinary 17-ketosteroid and serum androgen levels in patients with hirsutism. Am J Obstet Gynecol1977; 128: 408–17.
5.
FarberMMillanVGTurksayRNMitchellGWJrDiagnostic evaluation of hirsutism in women by selective bilateral adrenal and ovarian venous catheterisation. Fertil Steril1978; 30: 283–8.
6.
TanSYDonabedianRGenelMMalrowPJ. False elevation of plasma cortisol in congenital adrenal hyperplasia. J Lab Clin Med1977; 89: 735–40.
7.
SchoneshoferMFennerADulceHJ. Interferences in the radioimmunological determination of free urinary cortisol. Acta Endocrinol (Suppl)1979; 225: 56.
8.
JohnsonDWPhillipouGRalphMMSeamarkRF. Specific quantitation of urinary progesterone by gas chromatography-mass spectrometry. Clin Chim Acta1979; 94: 207–8.
9.
JohnsonDWPhillipouGJamesSKSeabornCJRalphMM. Specific quantitation of plasma dehydroepiandrosterone-sulphate by GC–MS: Comparison with a direct RIA. Clin Chim Acta1980; 106: 99–101.
10.
PhillipouGFrithRG. Specific quantitation of plasma medroxyprogesterone acetate by gas chromatography/mass spectrometry. Clin Chim Acta1980; 103: 129–133.
11.
LawsonAMMitchellFL. Plans for control of accuracy in hormone analysis. Clin Chem1977; 23: 916.
12.
PhillipouGSeamarkRFCoxLW. A procedure for comprehensive analysis of neutral urinary steroids in endocrine investigations. Aust NZ J Med1978; 8: 63–8.
13.
ShackletonCHLTaylorNFHonourJW. An atlas of gas chromatographic profiles of neutral urinary steroids in health and disease.Delft: Packard-Becker BV, 1980.
14.
PhillipouGJamesSKFrithRG. Urinary steroid levels in normal women in the follicular phase of the menstrual cycle. IRCS Med Sci1979; 7: 297.
15.
PhillipouGMcIntoshJEABighamDAFrithRGJamesSK. Statistical evaluation of urinary steroid measurements in normal, infertile and amenorrhoeic women. IRCS Med Sci1979; 7: 363.
16.
PhillipouGKirkJ.Significance of steroid measurements in male pattern alopecia. Clin Exp Dermatol in press.
17.
CheekDBGreystoneJESeamarkRFMcIntoshJEAPhillipouGCourtJM. Urinary steroid metabolites and the overgrowth of lean and fat tissues in obese girls. Am J Clin Nutr in press.
KovatsESz. Gas chromatography characterization or organic substances in the retention index system. Adv Chromatogr1965; 1: 229–47.
20.
BarrettSABrownJB. An evaluation of the method of Cox for the rapid analysis of pregnanediol in urine by gas-liquid chromatography. J Endocrinol1970; 47: 471–80.
21.
MetcalfMG. Dehydroepiandrosterone-sulphate in urine. Clin Biochem1978; 11: 65–9.
22.
LoboRAPaulWGoebelsmannU.Clinical use of plasma dehydroepiandrosterone sulphate: Correlations with total and fractionated urinary 17-ketosteroids. Fertil Steril1979; 32: 499.
TagatzGEGurpideE.Hormone secretion by the normal ovary. In: GreepRAstwoodE, eds. Handbook of physiology: endocrinology.Baltimore: Williams & Wilkins, 1973; 603–13.
