A simple and improved method of continuous-flow analysis for total and direct bilirubin in serum is described. Advantages of the method are: small serum and reagent volumes; the use of less concentrated caffeine solution in the accelerator; the substitution of distilled water for hydrochloric acid in the direct bilirubin channel; and the close agreement of results obtained by the present and Jendrassik-Grof reference method when purified bilirubin is used as the calibrating standard.
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