A novel, solid phase, enzyme immunoassay for the measurement of human plasma follicle stimulating hormone has been developed. The method is a direct, competitive inhibition system of high precision and accuracy. It compares favourably with solid phase radioimmunoassay using the same antiserum and hormone preparations. Hormone reference values are presented for normal men, and for women at various stages in the menstrual cycle and in the menopausal state.
References
1.
AvrameasS. (1969). Coupling of enzymes to proteins with glutaraldehyde. Immunochemistry, 6, 43–52.
2.
AvrameasS.TernynckT. (1971). Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry, 8, 1175–1179.
3.
DixonM.WebbE. (1965). In Enzymes, chapter 10, p. 454. Longmans.
HumB.LandonJ. (1971). In Radioimmunoassay Methods, p. 124. Churchill Livingstone.
7.
LoveseyA. (1980). A rapid solid phase radioimmunoassay for human plasma follicle stimulating hormone. Annals of Clinical Biochemistry, 17 (in Jan. issue).
8.
WhitakerJ. (1963). Determination of molecular weights of proteins by gel filtration on Sephadex. Annalytica Chemistry, 35, 1950–1953.
9.
WideL. (1973). Radioimmunoassay of FSH and LH in serum and urine from men and women. Acta Endocrinologia, Supplement, 174, 1–60.