Separation of Creatine from Arginine in Biological Fluids by Thin-Layer Chromatography

Abstract

Creatine in biological fluids was estimated using a modified Sakaguchi procedure. Interference by arginine was eliminated by thin-layer chromatography.

References

Anderson

D. R.

Williams

C. M.

Krise

G. M.

Dowben

R. M.

(1957). Determination of creatine in biological fluids. Biochemical Journal, 67, 258.

Sakaguchi

(1925). A new color reaction of protein and arginine. Journal of Biochemistry, 5, 25.

Satake

Luck

J. M.

(1958). The spectrophotometric determination of arginine by Sakaguchi reaction. Bulletin de la Societé de Chimie Biologique, 40, 1943.

Vogel

A. I.

(1958). Miscellaneous reactions. In Text Book of Practical Organic Chemistry, 3rd edition, p. 926, Longmans, London.