An automated kinetic assay for the determination of glucose in blood is described. The method employs the enzyme glucose dehydrogenase in the presence of mutarotase, with nicotinamide adenine dinucleotide as hydrogen acceptor. The analytical parameters of the method are determined and the flexibility of the method in relation to sample volume and sensitivity is discussed. Finally, the method is compared with automated glucose oxidase and hexokinase procedures.
References
1.
BanauchD.BrümmerW.EbelingW.MetzH.RingfreyH.LangH.LeyboldK.RickW. (1975). Eine glucose Dehydrogenase für die Glucose-Bestimmung in Körper-flüssigkeiten. Zeitschrift für klinische Chemie und Biochemie, 13, 101–107.
2.
CrampD. G. (1970). Automated fluorimetric technique based on NAD and NADP linked enzyme systems in the study of intermediary carbohydrate metabolism. Journal of Medical Laboratory Technology, 27, 359–382.
3.
GutteridgeJ. M.WrightE. B. (1968). A simple automated guaiacum glucose-oxidase method. Journal of Medical Laboratory Technology, 25, 385–386.
4.
KuanJ. W.KuanS. S.GuilbaultG. G. (1977). Determination of plasma glucose with use of a stirrer containing immobilized glucose dehydrogenase. Clinical Chemistry, 23, 1058–1060.
5.
LutzR. A.FlückigerJ. (1975). Kinetic determination of glucose with the GEMSAEC (ENI) centrifugal analyser by the glucose dehydrogenase reaction, and the comparison with two commonly used procedures. Clinical Chemistry, 21, 1372–1376.
6.
PardueH. (1977). A comprehensive classification of kinetic methods of analysis in clinical chemistry. Clinical Chemistry, 23, 2189–2201.
7.
PasseyR. B.GillumR. L.FullerJ. B.UrryF. M.GilesM. L. (1977). Evaluation and comparison of 10 glucose methods and the reference method recommended in the proposed Product Class Standard (1974). Clinical Chemistry, 23, 131–139.
8.
PaulyH. E.PfleidererG. (1975). D-glucose dehydrogenase from Bacillus megaterium M1286: purification, properties and structure. Hoppe Seyler's Physiological Chemistry, 356, 1613–1618.
9.
PriceC. P.LloydB.AlbertiK. G. M. M. (1977). A kinetic spectrophotometric assay for rapid determination of acetoacetate in blood. Clinical Chemistry, 23, 1893–1897.
