Summary A method of acetate determination by gas phase chromatography using a porous polymer stationary phase is reported that is suitable for use with aqueous and plasma samples. It is linear up to 100 mmol/l and has a coefficient of variation of less than 4 % for acetate values of greater than 1 mmol/l. The recovery of acetate from plasma is 92 % and sample retention time is 3 minutes. The reference range of plasma acetate in a group of 40 apparently healthy subjects was from less than 0·1 to 0·35 mmol/l. The frequently encountered problem of adsorption and ghosting of volatile fatty acids is overcome without the addition of formic acid vapour to the carrier gas.
References
1.
AckmanR. G. (1972). Porous polymer bead packings and formic acid vapour in the GLC of volatile free fatty acids. Journal of Chromatographic Science, 10, 560–565.
2.
BergmeyerH. U.MölleringH. (1974). Acetate. In Methods of Enzymatic Analysis, 3rd edition, volume 3, edited by BergmeyerH. U., p. 1520. Academic Press, New York.
3.
DeschG.DescompsB. (1977). Dosage rapide par chromatographie en phase gazeuse de l'acétate dans le plasma et les bains d'hémodialyse de suppléance. Clinica Chimica Acta, 76, 193–204.
4.
EarnestD. L.SandlerJ. H.IngramR. H.MaconE. J. (1968). Acid base balance in chonic hemodialysis. Transactions. American Society for Artificial Internal Organs, 14, 434–437.
5.
GeddesDorothy A. M.GilmourMarion N. (1970). The control of ghosting, a major source of error in gas liquid chromatographic determinations of C2-C5 acids. Journal of Chromatographic Science, 8, 394–397.
6.
GraefeU.MilutinovichJ.FoletteW. C.BabbA. L.ScribnerB. H. (1977). Improved tolerance to rapid ultra filtration with use of bicarbonate in dialysate. Proceedings of the European Dialysis and Transplant Association, 14, 153–159.
7.
KveimM.BredersonJ. E. (1977). A gas chromatographic method for determination of acetate levels in body fluids. Scandinavian Journal of Clinical and Laboratory Science (In press).
8.
KveimM.NesbakkenR. (1975). Utilisation of exogenous acetate during hemodialysis. Transactions. American Society for Artificial Internal Organs, 21, 138–143.
9.
LundquistF. (1962). Production and utilisation of free acetate in man. Nature, 193, 579–580.
10.
LundquistF.TygstrupN.WinklerK.MellemgaardK.Munck-PetersenS. (1962). Ethanol metabolism and production of free acetate in the human liver. Journal of Clinical Investigation, 41, 955–961.
11.
MansellM. A.WingA. J. (1976). Long term experience of home dialysis with sorbent regeneration of dialysate. Proceedings of the European Dialysis and Transplant Association, 13, 275–283.
12.
MionC. M.HegstromR. M.BoenS. T.ScribnerB. H. (1964). Substitution of sodium acetate for sodium bicarbonate in the bath fluid for hemodialysis. Transactions. American Society for Artificial Internal Organs, 10, 110–113.
13.
NovelloA.KelschR. C.EasterlingR. E. (1976). Acetate intolerance during hemodialysis. Clinical Nephrology, 5, 29–32.
14.
OttensteinD. M.BartleyD. A. (1971). Separation of free acids C2-C6 in dilute aqueous technology. Journal of Chromatographic Science, 9, 673–681.
15.
PortF. K.EasterlingR. E. (1975). Evaluation of acetate tolerance during highly efficient hemodialysis. Proceedings of the Dialysis Transplant Forum, 5, 128–130.
16.
RosenbergL. E. (1972). Disorders of propionate, methyl-malonate and vitamin B12 metabolism. In Inherited Basis of Metabolic Disease, 3rd edition, edited by StanburyJ. B.WyngaardenJ. B.FredericksonD. S., chapter 22. McGraw-Hill, New York.
17.
ScribnerB. H. (1977). Substitution of bicarbonate for acetate in the dialysate for care of a critically ill patient. Dialysis and Transplantation, 6, 26.
18.
StokkeO.JellumE.EldjarnL.SchnitlerR. (1973). The occurrence of ξ-hydroxy-η-valeric acid in a patient with propionic and methylmalonic acidaemia. Clinica Chimica Acta, 45, 391–401.
19.
WadkeM.LowensteinJ. M. (1975). Determination of acetate by gas-liquid chromatography. Methods in Enzymology, 35, 307–311.
20.
WhitfieldJ. B. (1969). Metabolic effects of hemodialysis against glucose and acetate containing solutions. Clinica Chimica Acta, 24, 449–452.
