During the course of a kinetic enzyme study of bisphosphoglyceromutase (EC 2.7.5.4.) a chemiluminescent effect was encountered in the liquid scintillation counting of the β-emission from 32Pi. A method is described for overcoming the effect, thereby permitting the accurate determination of the radioactive compounds. It is important to check for chemiluminescent effects early in the development of new procedures that involve liquid scintillation counting.
References
1.
HerbergR. J. (1960). Determination of carbon-14 and tritium in blood and other whole tissues. Analytical Chemistry, 32, 42–46
2.
KahlbehnD. A. (1965). Problems of chemiluminescence in liquid scintillation counting using the hydroxide of hyamine 10-X. International Journal of Applied Radiation and Isotopes, 18, 655–656
3.
NegeleinE. (1957). Methods in Enzymology, volume 3, p. 216. Edited by ColowickS. P.KaplanN. O.. Academic Press: London
4.
RapkinE. (1968). Preparation of Samples for Liquid Scintillation Counting. Monograph. Picker Nuclear Corporation: Cleveland
5.
RoseZ. B. (1968). The purification and properties of diphosphoglycerate mutase from human erythrocytes. Journal of Biological Chemistry, 243, 4810–4820
