A new method of separating serum or plasma from whole blood (Sure-Sep, W. R. Warner) has been evaluated with particular regard to the suitability of the serum/plasma samples for radioimmunoassay (R.I.A.) and competitive protein binding (C.P.B.) techniques. Sure-Sep did not affect the results obtained for the following assays: cortisol, thyroxine, triidothyronine, insulin, and growth hormone. In addition it was also found that contact of the serum or plasma samples with Sure-Sep for 24 hours at 4°C was without effect on the results obtained.
References
1.
BrodalB. P.Evidence of an enzymatic degradation of insulin in bloodin vitro. Europ. J. Biochem.18 (1971) 201
2.
HalesC. N.RandleP. J.Immunoassay of insulin with insulin-antibody precipitate. Biochem. J.88 (1963) 137
3.
MathiesJ. C.Evaluation of a new device for rapidly separating serum or plasma from blood. Clin. Chem.20 (1974) 1573