Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destruction of oxygen free radicals in white blood cells, red blood cells, and subcellular particles are discussed, (a) The production of superoxide anions by white cells during phagocytosis is thought to be advantageous for the overall bactericidal event. (b) Normal red blood cells generate low levels of superoxide anions. Increased levels of free-radical production could play a significant role in accelerating cell ageing (haemolysis), (c) Subcellular particles produce superoxide anions. These as well as organic peroxides have been implicated in drug hydroxylation reactions involving cytochrome P-450.
References
1.
AustS. D.RoerigD. L.PedersonT. C.Evidence or superoxide generation by NADPH-cytochrome C reductase of rat liver microsomes. Biochem. biophys. Res. Commun., 47 (1972) 1133.
2.
BabiorB. M.KipnesR. S.CurnutteJ. T.The production by leucocytes of superoxide. A potential bactericidal agent, J. clin. Invest., 52 (1973) 741.
3.
BeauchampC.FridovitchI.Superoxide dismutase; improved assays and an assay applicable to acrylamide gels. Analyt. Biochem., 44 (1971) 276.
4.
CarrellR. W.WinterbournC. C.RachmilewitzE. A.Activated oxygen and haemolysis (Annotation). Brit. J. Haemat., 30 (1975) 259.
5.
CarricoR. J.DeutschH. F.The presence of zinc in human cytocuprein and some properties of the apoprotein. J. biol. Chem., 245 (1970) 723.
CurnutteJ. T.BabiorB. M.The effect of bacteria and serum on superoxide production by granulocytes. J. clin. Invest.53 (1974) 1662.
8.
CurnutteJ. T.WhittenD. M.BabiorB. M.Defective superoxide production by granulocytes from patients with chronic granulomatous disease. New Engl. J. Med.290 (1974) 593.
DodgeJ. T.CohenG.KaydenH. J.PhillipsG. B.Peroxidative haemolysis of red blood cells from patients with Abeta lipoproteinemia (Acanthocytosis). J. clin. Invest.46 (1967) 357.
11.
FridovitchI. In Advances in Enzymology, Vol. 41, Ed. Meister, London, Interscience, 1974.
12.
GregoryE. M.FridovitchI.Oxygen toxicity and the superoxide dismutase. J. Bact.114 (1973) 1193.
13.
GrossR. T.BracciR.RudolphN.SchroederE.KochenJ. A.Hydrogen peroxide toxicity and detoxification in the erythrocytes of the new born infants. Blood29 (1967) 381.
14.
GutteridgeJ. M. C.Consequences of lipid autoxidation in biological material. Ph.D. Thesis, London University, 1974.
15.
GutteridgeJ. M. C.LamportP.DormandyT. L.Autoxidation as a cause of antibacterial activity in unsaturated fatty acids. J. med. Microbiol.7 (1974) 387.
16.
GutteridgeJ. M. C.LamportP.DormandyT. L.The antibacterial effect of water-soluble compounds from autoxidising linolenic acid. J. med. Microbiol.9 (1976) 105.
17.
HarberF.WeissJ.The catalytic decomposition of hydrogen peroxide by iron salts, Proc. roy. Soc. A146 (1934) 332.
18.
HolmesB.PageA. R.GoodR. A.Studies of the metabolic activity of leucocytes from patients with a genetic abnormality of phagocytic function. J. clin. Invest.46 (1967) 1422.
19.
HolmesB.ParkB. H.MalawistaS. E.QuieP. G.NelsonD. L.GoodR. A.Chronic granulomatous disease in females: A deficiency of leucocyte glutathione peroxidase. New Engl. J. Med.283 (1970) 217.
20.
HrycayE. G.O'BrienP. J.Cytochrome P-450 as a microsomal peroxidase in steroid hydroperoxide reduction. Arch. Biochem. Biophys.153 (1972) 480.
21.
JacobH. S.LuxS. E.Degradation of membrane phospholipids and thiols in peroxide hemolysis: Studies in Vitamin E deficiency. Blood32 (1968) 549.
22.
JohnstonR. B.Jr.KeeleB. B.Jr.MisraH. P.LehmeyerJ. E.WebbL. S.BaehnerR. L.RajogopalanK. V.The role of superoxide anion generation in phagocytic bactericidal activity. J. clin. Invest.55 (1975) 1357.
KeeleB. B.Jr.McCordJ. M.FridovitchI.Superoxide dismutase from Escherichia coli B, a new manganese containing enzyme. J. biol Chem.245 (1970) 6176.
25.
KimmelJ. R.MarkowitzH.BrownD. M.The isolation of the copper-containing protein cerebrocuprein from normal human brain. J. biol. Chem.234 (1959) 46.
26.
LamolaA. A.YamaneT.TrozzoloA. M.Cholesterol hydroperoxide formation in red cell membranes and photohemolysis in erythropoietic protoporphyria. Science179 (1973) 1131.
27.
LittleG.O'BrienP. J.Effectiveness of a lipid peroxide in oxidising proteins and nonprotein thiol. Biochem. J.106 (1967) 419.
28.
LorberA.CutlerL. S.ChangC. C.Serum copper levels in rheumatoid arthritis. Relationship of elevated copper to protein alterations. Arthr. and Rheum.11 (1968) 65.
29.
MannT.KeilinD.Haemocruprein and hepatocuprein. Copper-protein compounds of blood and liver of mammals, Proc. roy. Soc. B126 (1938) 303.
30.
MengelC. E.KannH. E.SmithW.HortonB.Effects of in vivo hyperoxia on erythrocytes. Hemolysis in mice exposed to hyerbaric oxygenation. Proc. Soc. exp. Biol. (N.Y.)116 (1964) 259.
31.
MengelC. E.KannH. E.Jr.Effects of in vivo hyperoxia on erythrocytes. III. In vivo peroxidation of erythrocyte lipid. J. clin. Invest.45 (1966) 1150.
32.
McCordJ. M.FridovitchI.Superoxide dismutase, an enzymic function for erythrocuprein (haemocuprein). J. biol. Chem.244 (1969) 6049.
33.
McCordJ. M.Free radicals and inflammation: Protection of synovial fluid by superoxide dismutase. Science185 (1974) 529.
34.
MisraH. P.FridovitchI.The generation of superoxide radical during the autoxidation of hemoglobin. J. biol. Chem.247 (1972) 6960.
35.
NitowskyH. M.TildonJ. T.Some studies of tocopherol deficiency in infants and children. III. Relation of blood catalase activity and other factors to hemolysis of erythrocytes and hydrogen peroxide. Amer. J. clin. Nutr.4 (1956).
36.
PorterH.AinsworthS.The isolation of the copper-containing protein cerebrocuprein from normal human brain. J. Neurochem.5 (1959) 91.
37.
PorterH.SweeneyM.PorterE. M.Human hepatocuprein. Isolation of a copper-protein from the subcellular soluble fraction of adult human liver. Arch. Biochem.105 (1964) 319.
RäihäN.Hemolysis of human blood caused by oxygen and its prevention with Vitamin E. Acta paediat. (Uppsala)44 (1955) 128.
40.
SalinM. L.McCordJ. M.Free radicals and inflammation: Protection of phagocytosing leukocytes by superoxide dismutase. J. clin. Invest.56 (1975) 1319.
41.
SerfassR. E.GantherH. E.Defective microbiocidal activity in glutathione peroxidase—deficient neutrophils of selenium—deficient rats. Nature (Lond.)255 (1975) 640.
42.
ShohetS. B.PittJ.BaehnerR. L.PoplackD. G.Lipid peroxidation in the killing of phagocytized pneumococci. Infect. Immunity10 (1974) 1321.
43.
SlaterT. F.Free Radical Mechanisms in Tissue Injury.London, Pion, 1972.
44.
StocksJ.DormandyT. L.The autoxidation of human red cells Lipids induced by hydrogen peroxide. Brit. J. Haemat.20 (1971) 95.
45.
StocksJ.OffermanE. L.ModellC. H.DormandyT. L.The susceptibility to autoxidation of human red cell lipids in health and disease. Brit. J. Haemat.23 (1972) 713.
46.
StocksJ.GutteridgeJ. M. C.SharpR.DormandyT. L.Assay using brain homogenate for measuring the antioxidant activity of biological fluids. Clin. Sci. mol. Med.47 (1974) 215.
47.
StocksJ.GutteridgeJ. M. C.SharpR.DormandyT. L.The inhibition of lipid autoxidation by human serum and its relation to serum proteins and tocopherol. Clin. Sci. mol. Med.43 (1974) 223.
48.
StocksJ.ScudderP.GutteridgeJ. M. C.DormandyT. L. (1976). To be published.
49.
StosselT. P.MasonR. J.SmithA. L.Lipid peroxidation by human blood phagocytes. J. clin. Invest.54 (1974) 638.
50.
StrobelH. W.CoonM. J.Effect of superoxide generation and dismutation on hydroxylation reactions catalysed by liver microsomal cytochrome P—450. J. biol. Chem.246 (1971) 7826.
51.
WeisigerR. A.FridovitchI.Superoxide dismutase. Organelle specificity. J. biol. Chem.248 (1973) 3582.
52.
YostF. J.Jr.FridovitchI.An iron-containing superoxide dismutase from Escherichia coli. J. biol. Chem.248 (1973) 4905.
53.
ZimmermanR.FloheL.WeserN.HartmanH. J.Inhibition of lipid peroxidation in isolated inner membranes of rat liver mitochrondia by superoxide dismutase. FEBS left.29 (1973) 117.
