The effects of venestasis and hand exercise on antecubital vein blood lactate and pyruvate concentrations have been re-examined. The results show that venestasis for up to 5 min has no effect, while hand exercise for as little as 20 s causes a marked increase in blood lactate and pyruvate concentrations. The implications of these findings are discussed.
References
1.
CrampD. G.Automated fluorimetric techniques based on NAD/NADP linked enzyme systems in the study of intermediary carbohydrate metabolism. J. med. Lab. Technol.29 (1970) 359.
2.
DrewesP. A. In Clinical Chemistry: Principles and Technics, ed. HenryR. J.CannonD. C.WinkelmanJ. W. (1974) p. 1331. Hagerstown, Harper and Row.
3.
FriedemannT. E.HaugenG. E.Collection of blood for the determination of pyruvic and lactic acids. J. biol. Chem.144 (1942) 62.
4.
FriedemannT. E.HaugenG. E.KmieciakT. C.The level of pyruvic and lactic acids, and the lactic/pyruvic ratio, in the blood of human subjects. The effect of food, light muscular activity, and anoxia at high altitude. J. biol. Chem.157 (1945) 673.
5.
MendelB.EngelW.GoldscheiderI.Über den Milchsäuregehalt des Blütes unter physiologischen und pathologischen Bedingungen. Klin. Wschr.4 (1925) 306.
6.
SegalS. S.BlairA. E.WyngaardenJ. B.An enzymatic spectrophotometry method for the determination of pyruvic acid in blood. J. Lab. clin. Med.48 (1956) 137.