A comparison is made of the accuracy, precision, and convenience of two manual serum triglyceride methodologies based on solid phase adsorption and liquid phase partition procedures. Both methods are accurate but the precision and convenience of the latter is far superior.
The use of home made reagents reduces the cost of both substantially below that of any commercial alternative, including the popular enzymatic methods.
References
1.
BellJ. L.AtkinsonS. M.BaronD. N.An Autoanalyzer method for estimation of serum glyceride glycerol using a glycerokinase procedure. J. clin. Path.23 (1970) 509.
KesslerG.LedererH.Fluorometric measurement of triglycerides. Technicon Symposium, Automation in Analytical Chemistry, New York, (1965) p. 341.
10.
LevyA. L.KeylounC.An automated colorimetric (non-fluorometric) assay for triglycerides. Technicon Symposium, Automation in Analytical Chemistry, New York (1970) p. 497.
11.
LoflandH. B.A semi-automated procedure for the determination of triglycerides in serum. Ann. Biochem.9 (1964) 393.
NashT.The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J.55 (1953) 416.
14.
NeillD. W.RosalkiS. B.WildingP.A survey on the use of kits in British clinical chemistry laboratories.Association of Clinical Biochemists, London (1973).
15.
NobleR. P.CampbellF. K.Improved accuracy in automated fluorometric determination of plasma triglycerides. Clin. Chem.16 (1970) 166.
16.
RoyerM. E.KoH.A simplified semi-automated assay for plasma triglycerides. Ann. Biochem.29 (1969) 405.
17.
RushR. L.LeonL.TurrellJ.Automated simultaneous cholesterol and triglycerides determination on the Autoanalyzer II instrument. Technicon Symposium, Automation in Analytical Chemistry, New York (1970) p. 503.
18.
SoloniF. G.Simplified manual micromethod for determination of serum triglycerides. Clin. Chem.17 (1971) 529.
19.
van HandelE.ZilversmitD. B.Micromethod for direct determination of serum triglycerides. J. Lab. clin. Med.50 (1957) 152.