Automated Two-Dimensional Immunoelectrophoresis and its Application to the Analysis of C3 and C4 in Rheumatoid Arthritis and Systemic Lupus Erythematosus (S.L.E.)

Abstract

A semi-automated two-dimensional immunoelectrophoretic method has been developed which is quicker and cheaper to use than the original system, making it more suitable for routine use. It is accurate and reproducible and is suitable for complement conversion studies where C3 and C4 conversion can be measured with 5 and 10% reproducibility respectively. Conversion of C3 and C4, observed in both S.L.E. and rheumatoid arthritis, is an early and sensitive index of disease activity. Total C3 levels, although fluctuating widely in the course of the disease, were not shown to be as sensitive an index.