A modification of Broughton's method for the determination of barbiturate in biological fluids is described which reduces the time of analysis to 15 min. and requires only 2 ml blood. Minimal reagents and apparatus are required. The method has been standardised and is accurate to barbiturate concentrations as low as 0.5 mg/100 ml blood.
References
1.
BroughtonP. M. G.A rapid ultraviolet spectrophotometric method for the detection, estimation and identification of barbiturates in biological material. Biochem. J.63 (1956) 207.
2.
GoldbaumL. R.Determination of barbiturates. Anal. Chem.24 (1952) 1604.
