Phospholipids from the red cells of 85 children, both normal and belonging to various clinical groups, have been extracted and subjected to silica gel chromatography. The several phospholipids have been quantitated. The results have not proved to be useful in diagnosis and hence do not confirm earlier reports. The problems associated with analysis of phospholipids by this type of method are discussed.
DodgeJ. T.PhillipsG. B.Autoxidation as a cause of altered lipid distribution in extracts from human red cells. J. Lipid Res. 7 (1966) 387.
3.
DodgeJ. T.PhillipsG. B.Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells. J. Lipid Res. 8 (1967) 667.
4.
FangM.MarinettiG. V.Chromatography of lipids on silica-gel-loaded filter paper. Methods in Enzymology14 (1969) 598. Ed. J. M. Lowenstein, Academic Press, New York.
5.
FarquharJ. W.AhrensE. H.Jr.Effects of dietary fats on human erythrocyte fatty acid patterns. J. Clin. Invest.42 (1963) 675.
6.
FleisherS.RouserG. Lipids of subcellular particles. J. Amer. Oil Chem. Soc.42 (1965) 588.
7.
GomoriG. A modification of the colorimetric phosphorus determination for use with the photoelectric colorimeter. J. Lab. Clin. Med.27 (1942) 955.
8.
GriffithsM. I.JohnF. M.The value of red cell phospholipid determination in the diagnosis of mental with physical handicap in children. Arch. Dis. Childh. (1973) In the press.
9.
HanahanD. J.WattsR. M.PappajohnD. Some chemical characteristics of the lipids of human and bovine erythrocytes and plasma. J. Lipid Res. 1 (1960) 421.
10.
HooghwinkelG. J. M. (1971). Private communication.
11.
HooghwinkelG. J. M.BorriP. F.BruynG. W.Biochemical studies in Huntington's chorea. II. Composition of blood lipids. Acta Neurol. Scand.42 (1966) 213.
12.
HooghwinkelG. J. M.van GeldernH. H.StaalA. Sphingomyelin of red blood cells in lipidosis and in dementia of unknown origin in children. Arch. Dis. Childh.44 (1969) 197.
13.
JaffeE. R.GottfriedE. L.Hereditary nonspherocytic hemolytic disease associated with an altered phospholipid composition of the erythrocytes. J. Clin. Invest.47 (1968) 1375.
14.
KatesM. Paper chromatography of phosphatides and glycolipids on silicic-acid-impregnated filter paper. Lipid Chromatographic Analysis, Vol. 1 (1967) p. 31. Ed. MarinettiG. V., Marcel Dekker, Inc., New York.
15.
MarinettiG. V.Chromatographic separation, identification, and analysis of phosphatides. J. Lipid Res. 3 (1962) 1.