The determination of urinary hormones is useful in the assessment of gonadal and pituitary function. For interpretation of these parameters it is essential to establish normal values. This paper describes some of the methods used and results obtained in the determination of urinary pregnanediol, oestrogens, L.H. and F.S.H. during a number of normal cycles.
References
1.
BarrettS. A.BrownJ. B.An evaluation of the method of Cox for the rapid analysis of pregnanediol in urine by gas-liquid chromatography. J. Endocrinol.47 (1970) 471.
2.
BrownJ. B.MacLeodS. C.MacNaughtonC.SmithM. A.SmythB. A rapid method for estimating oestrogens in urine using a semi-automatic extractor. J. Endocrinol.42 (1968a) 5.
3.
BrownJ. B.MacNaughtonC.SmithM. A.SmythB. Further observations on the Kober colour and Ittrich fluorescence reactions in the measurement of oestriol, oestrone and oestradiol. J. Endocrinol.40 (1968b) 175.
4.
CoxR. I.The rapid analysis of pregnanediol as the free steriod. In Dunod (Ed.) Chromatographie en phase gazeuse, Gordon and Breach, New York, 1967, p. 309.