The optimal substrate, hydrogen ion, and coenzyme concentrations for Lactate Dehydrogenase and Alpha-Hydroxybutyrate Dehydrogenase have been established at the physiological temperature 37°C, using an LKB 8600 Reaction Rate Analyzer.
References
1.
International Union of Biochemistry. Enzyme Nomenclature. Elsevier, Amsterdam, 1965.
2.
International Union of Biochemistry. Report of the Commission on Enzymes. Pergamon Press, Oxford and London, 1961.
3.
BergmeyerH. U.The Standardisation of Enzymological Methods. Sixth International Congress of Clinical Pathology, Rome (1966).
4.
KingJ. Temperature standardisation in clinical enzymology. Clin. Biochem.1 (1967) 42.
5.
RosalkiS. B.WilkinsonJ. H.Reduction of α-ketobutyrate by human serum. Nature (Lond.)188 (1960) 1110.
6.
WroblewskiF.LaDueJ. S.Lactate dehydrogenase activity in blood. Proc. Soc. exp. Biol. (N.Y.)90 (1955) 210.
