The degree of glycation of plasma apolipoprotein A-I was measured by a combination of gel filtration, boronate affinity chromatography and latex immunoagglutination. The plasma concentrations of apolipoprotein A-I determined by this combination method (y) correlated well with those determined by turbidimetric immunoassay (x) (y= 1·12x + 1·9, r = 0·964). The inter- and intra-assay coefficients of variation in the glycated apolipoprotein A-I assay were 4·1–5·0% and 4·0–4·4%, respectively. Interference from plasma glucose at concentrations up to 55·1 mmol/L was eliminated by gel filtration. Labile glycated apolipoprotein A-I did not interfere with the measurement of glycated apolipoprotein A-I. Reference values for glycated apolipoprotein A-I were determined to be 2·4–4·0% (n = 140), with no significant difference between men and women. The mean concentration of plasma glycated apolipoprotein A-I in patients with uncontrolled diabetes mellitus (5·11%) was significantly higher than in normal subjects (3·12%, P < 0·001). The method is simple, rapid and highly sensitive for determination of the glycation level of plasma apolipoprotein A-I.
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