Often only small amounts of sample are available for spectroscopic analytical determinations. This work investigates the enhancement of signal in columns packed with silica particles. We propose that silica particles cause the light to scatter through the column, effectively increasing optical path length. Packed columns are shown to be effective with fluorescence spectroscopy, but results were inconclusive with absorbance spectroscopy.
NuernbergerP.KrampertG.BrixnerT.VogtG. “Rotation-Translation Device for Condensed-Phase Spectroscopy with Small Sample Volumes”. Rev. Sci. Instrum..2006; 77(8): 83113.
2.
YangT.StavrakisS.deMelloA. “A High-Sensitivity, Integrated Absorbance and Fluorescence Detection Scheme for Probing Picoliter-Volume Droplets in Segmented Flows”. Anal. Chem.2017; 89(23): 12880–12887.
3.
SongW.YangJ. “Optofluidic Differential Spectroscopy for Absorbance Detection of Sub-Nanolitre Liquid Samples”. Lab Chip.2012; 12(7): 1251–1254.
4.
PuleoC.M.YehH.C.LiuK.J.WangT.H. “Coupling Confocal Fluorescence Detection and Recirculating Microfluidic Control for Single Particle Analysis in Discrete Nanoliter Volumes”. Lab Chip.2008; 8(5): 822–825.
5.
AielloM.McLarenR. “A Sensitive Small-Volume UV/Vis Flow Cell and Total Absorbance Detection System for Micro-HPLC”. Anal. Chem.2001; 73(6): 1387–1392.
6.
BakerL.R.OrtonA.W.GoatesS.R.HornB.A. “Characterization of Carbon Dioxide Mobile Phase Density Profiles in Packed Capillary Columns by Raman Microscopy”. Appl. Spec.2009; 63(1): 108–111.
7.
BakerL.R.StarkM.A.OrtonA.W.HornB.A., et al.“Density Gradients in Packed Columns I: Effects of Density Gradients on Analyte Retention and Separation Speed”. J. Chromatogr. A.2009; 1216(29): 5588–5593.
8.
BakerL.R.OrtonA.W.StarkM.A.GoatesS.R. “Density Gradients in Packed Columns II: Effects of Density Gradients on Separation Efficiency”. J. Chromatogr. A.2009; 1216(29): 5594–5599.
9.
AronsonR.CorngoldN. “Photon Diffusion Coefficient in an Absorbing Medium”. J. Opt. Soc. Am. A.1999; 16(5): 1066–1071.
10.
SchmittJ.M.ZhouG.X.WalkerE.C.WallR.T. “Multilayer Model of Photon Diffusion in Skin”. J. Opt. Soc. Am. A.1990; 7(11): 2141–2153.
11.
CanpolatM.PekcanO. “Photon Diffusion Study in Films Formed from High-T Latex Particles”. Polymer.1995; 36(23): 4433–4438.
12.
SallenG.TribuA.AicheleT.AndreR., et al.“Subnanosecond Spectral Diffusion Measurement Using Photon Correlation”. Nat. Photonics.2010; 4(10): 696–699.
13.
MalikA.LiW.LeeM.L. “Preparation of Long Packed Capillary Columns Using Carbon Dioxide Slurries”. J. Microcolumn Sep.1993; 5(4): 361–369.
