5B. New Technology: Molecular Biology

Nelson MB Apicella MA Murphy TF Vankeulen H Spotila LD Rekosh D . Cloning and sequencing of Haemophilus influenzae outer membrane protein P6. Infect Immun 1988; 56: 128–34.

Deich RA Metcalf BJ Finn CW Farley JE Green BA . Cloning of genes encoding a 15,000–dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically-related 15,000–dalton protein from Haemophilus influenzae. J Bacterid 1988;170: 489–98.

Green BA Metcalf BJ Quinn-Dey T Kirkley DH Quataert SA Deich RA . A recombinant non-fatty acylated form of the HiPAL (P6) protein of Haemophilus influenzae elicits biologically active antibody against both nontypeable and type b H influenzae. Infect Immun 1990;58: 3272–8.

Murphy TF Kirkham C . Amino acid sequence of a surface-exposed epitope of the P6 outer membrane protein of nontypable Haemophilus influenzae. In: Lim DJ , ed. Abstracts of the Fifth International Symposium on Recent Advances in Otitis Media, Ft Lauderdale, Fla, 1991: 120–1.

Deich RA Anilionis A Fulginiti J . Antigenic conservation of the 15,000-dalton outer membrane lipoprotein PCP of Haemophilus influenzae and biologic activity of anti-PCP antisera. Infect Immun 1990; 58: 3388–93.

Munson R Jr Tolan RW Jr. Molecular cloning, expression, and primary sequence of outer membrane protein P2 of Haemophilus influenzae type b. Infect Immun 1989; 57: 88–94.

Hansen EJ Hasemann C Clausell A . Primary structure of the porin protein of Haemophilus influenzae type b determined by nucleotide sequence analysis. Infect Immun 1989; 57: 1100–7.

Munson R Jr Grass S . Purification, cloning, and sequence of outer membrane protein P1 of Haemophilus influenzae type b. Infect Immun 1988; 56: 2235–42.

Martin D Munson R Jr Grass S . Mapping of B-cell epitopes on the outer membrane P2 porin protein of Haemophilus influenzae by using recombinant proteins and synthetic peptides. Infect Immun 1991; 59: 1457–64.

Barenkamp S . Cloning, expression and DNA sequence analysis of genes for nontypable Haemophilus influenzae (NTHi) high molecular weight (HMW) outer membrane proteins which are targets of bactericidal antibody. In: Lim DJ Bluestone CD Klein JO Nelson JD Ogra PL , eds. Recent advances in otitis media. Proceedings of the Fifth International Symposium. Hamilton, Canada: Decker Periodicals, 1993: 379–80.

Kar S To SC-M Brinton CC . Cloning and expression in Escherichia coli of LKP pilus genes from a ontypeable Haemophilus influenzae strain. Infect Immun 1990; 58: 903–8.

Coleman T Grass S Munson R Jr. Molecular cloning, expression, and sequence of the pilin gene from nontypeable Haemophilus influenzae M37. Infect Immun 1991; 59: 1716–22.

Bakaletz LO Holmes KA Forney LJ Ahmed A Kolattukudy P Lim DJ . Cloning and sequencing of a pilin gene from NTHi strain #1128. In: Lim DJ , ed. Abstracts of the Fifth International Symposium on Recent Advances in Otitis Media, Ft Lauderdale, Fla, 1991: 127.

Gilsdorf JR Marrs CF McCrea KW Forney LJ . Cloning, expression, and sequence analysis of the Haemophilus influenzae type b strain M43p+ pilin gene. Infect Immun 1990; 58: 1065–72.

Janson H Heden LO Grubb A Ruan MR Forsgren A . Protein D, an immunoglobulin D-binding protein of Haemophilus influenzae: cloning, nucleotide sequence, and expression in Escherichia coli. Infect Immun 1991;59: 119–25.

Sorensen CH . Secretory immunoglobulins in the nasopharynx: implications in otitis media. In: Bernstein J Ogra P , eds. Immunology of the ear. New York, NY: Raven Press, 1987: 259–78.

Weiser JN Lindberg AA Manning EJ Hansen EJ Moxon ER . Identification of a chromosomal locus for expression of lipopolysaccharide epitopes in Haemophilus influenzae. Infect Immun 1989;57: 3045–52.

Weiser JN Love JM Moxon ER . The molecular mechanism of phase variation of H influenzae lipopolysaccharide. Cell 1989; 17: 657–65.

Weiser JN Maskell DJ Butler PD Lindberg AA Moxon ER . Characterization of repetitive sequences controlling phase variation of Haemophilus influenzae lipopolysaccharide. J Bacteriol 1990; 172: 3304–9.

Helander IM Linder B Brade H . Chemical structure of the lipopolysaccharide of Haemophilus influenzae strain 1–69 Rd–/b+. Description of a novel deep rough mutant. Eur J Biochem 1988; 15: 483–92.

Weiser JN Williams A Moxon ER . Phase-variable lipopolysaccharide structures enhance the invasive capacity of Haemophilus influenzae. Infect Immun 1990;58: 3455–7.

Samuelson A Borreli S Lindberg AA Gustavsson R Freijd A . Typing of non-encapsulated Haemophilus influenzae from infection-prone individuals using DNA-fingerprinting and lipopolysaccharide-specific monoclonal antibodies. In: Lim DJ Bluestone CD Klein JO Nelson JD Ogra PL , eds. Recent advances in otitis media. Proceedings of the Fifth International Symposium. Hamilton, Canada: Decker Periodicals, 1993: 383–5.

Mumtaz V Weiser JN Lindberg AA Moxon ER . Antigenic similarities in lipopolysaccharides of Haemophilus and Neisseria and expression of a digalactoside structure also present on human cells. Microb Pathogen 1990; 9: 441–50.

Needham CA . Haemophilus influenzae: antibiotic susceptibility. Clin Microbiol Rev 1988; 1: 218–27.

DeGraaff J Elwell OP Falkow S . Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol 1976; 126: 439–46.

Mendelman PM Chaffin DO Stull TL . Characterization of non-beta-lactamase-mediated ampicillin resistance in Haemophilus influenzae. Antimicrob Agents Chemother 1984;26: 235–44.

Malouin F Schryvers AB Bryan LE . Cloning and expression of genes responsible for altered penicillin-binding proteins 3a and 3b in Haemophilus influenzae. Antimicrob Agents Chemother 1987;31: 286–91.

Roberts MC Swenson CD Owens LM Smith AL . Characterization of chloramphenicol-resistant Haemophilus influenzae. Antimicrob Agents Chemother 1980;18: 610–5.

Ward J . Antibiotic-resistant Streptococcus pneumoniae: clinical and epidemiologic aspects. Rev Infect Dis 1981; 3: 254–66.

Klugman KP . Pneumococcal resistance to antibiotics. Clin Microbiol Rev 1990; 3: 171–96.

Hakenbeck R Tornette S Adkinson NF . Interaction of non-lytic b-lactams with penicillin binding proteins in Streptococcus pneumoniae. J Gen Microbiol 1987;133: 755–60.

Williamson R Tomasz A . Inhibition of cell wall synthesis and acylation of the penicillin-binding proteins during prolonged exposure of growing Streptococcus pneumoniae to benzyl- penicillin. Eur J Biochem 1985; 151: 475–83.

Dowson CG Hutchinson A Spratt BG . Extensive remodeling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae. Molec Microbiol 1989;3: 95–102.

Porras O Caugant DA Lagergard T Svanborg-Eden C . Application of multilocus enzyme gel electrophoresis to Haemophilus influenzae. Infect Immun 1986;53: 71–8.

Owen RJ . Chromosomal DNA fingerprinting — a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30: 89–99.

Loos BG Bernstein JM Dryja DM Murphy TF Dickinson DP . Determination of the epidemiology and transmission of nontypable Haemophilus influenzae in children with otitis media by comparison of total genomic DNA restriction fingerprints. Infect Immun 1989; 57: 2751–7.

Bernstein JM Dryja DM Loos BG Dickinson DP . Restriction fragment mapping of nontypable Haemophilus influenzae: a new tool to study this middle ear pathogen. Otolaryngol Head Neck Surg 1989; 100: 200–6.

Dickinson DP Loos BG Dryja DM Bernstein JM . Restriction fragment mapping of Branhamella catarrhalis: a new tool for studying the epidemiology of this middle ear pathogen. J Infect Dis 1988; 158: 205–8.

Ryan AF Baird A . Growth factors during proliferation of the middle ear mucosa. Acta Otolaryngol (Stockh) 1993; 113: 68–74.

Gendler SJ Burchell JM Duhig T . Cloning the cDNA coding for differentiation and tumour-associated mucin glycoproteins expressed by human mammary epithelium. Proc Natl Acad Sci USA 1987; 84: 6060–4.

Gum JR Byrd JC Hicks JW Toribara NW Lamport DTA Kim YS . Molecular cloning of human intestinal mucin cDNAs. J Biol Chem 1989; 264: 6480–7.

Gum JR Hicks JW Swallow DM . Molecular cloning of cDNAs derived from a novel human intestinal mucin gene. Biochem Biophys Res Commun 1990; 171: 407–14.

Lan MS Batra SK Qi W-N Metzgar RS Hollingsworth MA . Cloning and sequencing of human pancreatic tumor mucin cDNA. J Biol Chem 1990; 265: 15294–9.

Gerard C Eddy RL Shows TB . The core polypeptide of cystic fibrosis tracheal mucin contains a tandem repeat structure. J Clin Invest 1990; 86: 1921–7.

Porchet N Cong NV Dufosse J . Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairs. Biochem Biophys Res Commun 1991; 175: 414–22.

Geng J-G Bevilacqua MP Moore KL . Rapid neutrophil adhesion to activated endothelium mediated by GMP-140. Nature 1990; 343: 757–60.

Bevilacqua MP Stengelin S Gimbrone MA Seed B . Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 1989; 243: 1160–5.

Siegelman M van de Rijn M Weissman IL . Mouse lymph node homing receptor cDNA clone encodes a glycoprotein revealing tandem interaction repeats. Science 1989; 243: 1165–72.

Lasky L Yednock T Singer M . Cloning of a lymphocyte homing receptor reveals a lectin domain. Cell 1989; 56: 1045–55.