The reverse transcriptase—polymerase chain reaction and the nested polymerase chain reaction were used for detection of respiratory syncytial virus (RSV) sequences in middle ear effusions collected from children with otitis media. Sequences of RSV were detected in 21 of 34 samples tested. These samples were collected during and/or after natural outbreaks of RSV infection in the community. In those patients from whose nasopharynges RSV was isolated, the viral sequences were highly detectable (75%) in the effusions. These observations suggest RSV as an important factor in the pathogenesis of otitis media with effusion.
BernsteinJM. Recent advances in otitis media with effusion. Ann Allergy1985; 55: 544–51.
2.
TeeleDWKleinJORosnerBA. Epidemiology of otitis media in children. Ann Otol Rhinol Laryngol1980; 89(suppl 68): 5–6.
3.
HendersonFWCollinerAMSanyalMA. A longitudinal study of respiratory viruses and bacteria in the etiology of acute otitis media with effusion. N Engl J Med1982; 306: 1377–83.
4.
ErlichHAGelfandDHSaikiRK. Specific DNA amplification. Nature1988; 331: 461–2.
5.
AureliusEJohanssonBSkoldenbergB. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. Lancet1991; 337: 189–91.
6.
ChomczynskiPSacchiN. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem1987; 162: 156–9.
7.
CollinsPLHuangYTWertzGW. Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proc Natl Acad Sci USA1984; 81: 7683–7.
8.
MufsonMABelsheRBOrvellCNorrbyE. Respiratory syncytial virus epidemics: Variable dominance of subgroups A and B strains among children, 1981–1986. J Infect Dis1988; 157: 143–8.
9.
LennetteEHSchmidtNJ. Diagnostic procedures for viral and rickettsial infections. New York, NY: American Public Health Association, 1969.
10.
LohEElliottJFCwirlaSLanierLLDavisMM. Polymerase chain reaction with single-sided specificity: Analysis of T cell receptor delta chain. Science1989; 243: 217–20.
11.
DunningAMTalmudPHumphriesSE. Errors in the polymerase chain reaction. Nucleic Acids Res1988; 16: 10393.
12.
VeltriRWSprinklePM. Secretory otitis media. An immune complex disease. Ann Otol Rhinol Laryngol1976; 85(suppl 25): 135–9.
13.
BernsteinJMBrentjensJVladutiuA. Are immune complexes a factor in the pathogenesis of otitis media with effusion?Am J Otolaryngol1982; 3: 20–5.
14.
DoyleWJTakaharaTFiremanP. The role of allergy in the pathogenesis of otitis media with effusion. Arch Otolaryngol1985; 111: 502–6.
15.
OphirDHahnTSchattnerAWallachDAvielA. Tumor necrosis factor in middle ear effusions. Arch Otolaryngol Head Neck Surg1988; 114: 1256–8.
16.
KimHWCancholaJGBrandtCD. Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. Am J Epidemiol1973; 89: 422–34.
17.
OkamotoYTsutsumiHKumarNSOgraPL. Effect of breast feeding on the development of anti-idiotype antibody response to F glycoprotein of respiratory syncytial virus in infant mice after post-partum maternal immunization. J Immunol1989; 142: 2507–12.
18.
HornMECGreggI. Role of viral infection and host factors in acute episodes of asthma and chronic bronchitis. Chest1973; 63(suppl): 44–8.
19.
BerglundBSalmivalliAToivanenP. Isolation of respiratory syncytial virus from middle ear exudates of infants. Acta Otolaryngol (Stockh)1966; 61: 474–87.
20.
WelliverRCKaulTNSunMOgraPL. Defective regulation of immune responses in respiratory syncytial virus infection. J Immunol1984; 133: 1925–30.
