Experimental Sendai Virus-Induced Labyrinthitis in Guinea Pigs

Abstract

Guinea pigs were inoculated with a Sendai virus into the scala tympani and subsequent pathological changes of the cochleas were investigated by electron microscopy. Replication of the virus was indicated by buddings at the endolymphatic surface and by the intracytoplasmic occurrence of filamentous substances, ie, nucleocapsids. Budding viruses or free virus particles observed in a series of our experiments were identified as Sendai viruses by means of the immunological labeling with ferritin. Viral lesions, which were defined as the pathological changes of the cells associated with virus multiplication, were found in eight cochleas out of 20. This study revealed that the early lesions of the cochleas infected by Sendai viruses were primarily confined to Reissner's membrane and the stria vascularis, but the sensory cells were not affected.

Keywords

cochlea electron microscopy immunoelectron microscopy Sendai virus viral labyrinthitis

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References

Lindsay

Davey

Ward

. Inner ear pathology in deafness due to mumps. Ann Otol Rhinol Larvngol 1960; 69: 918–35.

Lindsay

Hemenway

. Inner ear pathology due to measles. Ann Otol Rhinol Laryngol 1954; 63: 754–71.

Beal

Hemenway

Lindsay

. Inner ear pathology of sudden deafness. Arch Otolaryngol 1967; 85: 591–8.

Davis

Shurin

Johnson

. Experimental viral labyrinthitis. Nature 1975; 254: 329–31.

Davis

Johnson

. Experimental viral infections of the inner ear. I. Acute infections of the newborn hamster labyrinth. Lab Invest 1976; 34: 349–56.

Shimokata

Nishiyama

Ito

. Affinity of Sendai virus for the inner ear of mice. Infect Immunol 1977; 16: 706–8.

Kushida

. A new method for embedding in a low viscosity resin “Quetol 651.” J Electron Microsc 1974; 23: 197.

Hosaka

Ikeguchi

. Studies on the pleomorphism of HVJ virions. Virology 1966; 29: 205–21.

Robinson

TWE

Cureton

RJR

Heath

. The pathogenesis of Sendai virus infection in the mouse lung. J Med Microbiol 1968; 1: 89–95.

10.

Appell

Kovatch

Redecliff

Gerone

. Pathogenesis of Sendai virus infection of mice. Am J Vet Res 1971; 32: 1839–41.

11.

Blandford

Heath

. Studies on the immune response and pathogenesis of Sendai virus infection of mice. I. The fate of viral antigens. Immunology 1972; 22: 637–49.

12.

Mims

Murphy

. Parainfluenza virus Sendai infection in macrophages, ependyma, choroid plexus, vascular endothelium and respiratory tract of mice. Am J Pathol 1972; 70: 315–28.

13.

Wolinsky

Gilden

Rorke

. Experimental panencephalitis induced in suckling mice by parainfluenza 1(6/94) virus. III. Ultrastructural studies. J Neuropath Exp Neurol 1976; 35: 271–86.

14.

Tanaka

Iwasaki

Koprowski

. Experimental parainfluenza type 1 virus induced encephalopathy in the adult mouse. Am J Pathol 1975; 79: 335–46.

15.

Shimokata

Nishiyama

Ito

. Pathogenesis of Sendai virus infection in the central nervous system of mice. Infect Immunol 1976; 13: 1497–502.

16.

Compans

Holms

Dales

Choppin

. An electron microscopic study of moderate and virulent virus cell interactions of the parainfluenza virus SV5. Virology 1964; 30: 411–26.

17.

McLean

Doane

. The morphogenesis and cytopathology of bovine parainfluenza type 3 virus. J Gen Virol 1971; 12: 271–9.

18.

Tsai

Thomson

. Bovine parainfluenza type 3 virus infection: Ultrastructural aspects of viral pathogenesis in the bovine respiratory tract. Infect Immunol 1975; 11: 783–803.

19.

Fenner

White

. Medical virology. New York: Academic Press, 1976: 143–4.

20.

Kikuchi

Hilding

. The development of the stria vascularis in the mouse. Acta Otolaryngol (Stockh) 1966; 62: 277–91.