Natural killer (NK)-mediated cytotoxicity against the K562 cell line was evaluated in normal subjects and in patients with laryngeal cancer. Results showed reduced lymphocyte cytotoxicity in patients with neoplastic disease and further reduction in NK activity following postoperative radiation therapy. The authors discuss the possibility of using this test in the immunological monitoring of patients with laryngeal carcinoma.
Get full access to this article
View all access options for this article.
References
1.
HallerOKiesslingROrnAWigzellH. Generation of natural killer cells: An autonomous function of the bone-marrow. J Exp Med1977; 145: 1411–5.
2.
HerbermanRBHoldenHT. Natural cell-mediated cytotoxicity in nude mice. In: FoghJBoivanellaBC, eds. Nude mouse in experimental and clinical research. New York: Academic Press, 1978.
3.
VoseBMVankyFKleinE. Human tumor-lymphocyte interaction in vitro. V. Comparison of the reactivity of tumour-infiltrating, blood and lymph-node lymphocytes with autologous tumour cells. Int J Cancer1977; 20: 895–901.
4.
VoseBMVankyFKleinE. Lymphocyte cytotoxicity against autologous tumour biopsy cells in humans. Int J Cancer1977; 20: 512–5.
5.
GreenbergAHPlayfairJH. Spontaneously arising cytotoxicity to the P-815-Y mastocytoma in NZB mice. Clin Exp Immunol1974; 16: 99–104.
6.
GreenbergAHShenLWalkerLArnaiz-VillnaARoittIM. Characteristics of the effector cells mediating cytotoxicity against antibody-coated target cells. II. The mouse nonadherent K cells. Eur J Immunol1975; 5: 474–9.
7.
HerbermanRBNunnMEHoldenHPStaalSDjeuJY. Augmentation of natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic target cells. Int J Cancer1975; 16: 216–21.
8.
PuccettiPSantoniARiccardiCHerbermanRB. Cytotoxic effector cells with the characteristics of natural killer cells in the lungs of mice. Int J Cancer1980; 25: 153–9.
9.
FishingerPJNomuraS. Efficient release of murine xenotropic onco RNA-virus after murine leukemia virus infection of mouse cells. Virology1975; 65: 304–9.
10.
GlaserMHerbermanRB. Secondary cell mediated cytotoxic reaction against allogeneic lymphoma cells. J Natl Cancer Inst1976; 56: 1211–6.
11.
SatoHBoyseEAAokiTIritaniCOldLJ. Leukemia-associated transplantion antigens related to murine leukemia virus. J Exp Med1973; 138: 593–9.
12.
SenjdoFAokiTBoyseEABuafoGK. Natural occurrence of lymphocytes showing cytotoxic activity to BALB/C radiation induced leukemia RL81 cells. J Natl Cancer Inst1975; 55: 603–9.
13.
TakasugiMMickeiMRTerasakiPI. Reactivity of lymphocytes from normal persons on cultured tumor cells. Cancer Res1973; 33: 2898–904.
14.
KiesslingRKleinEWigzellH. Natural killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells: Specificity and distribution according to genotype. Eur J Immunol1975; 5: 112–7.
15.
GambergCGAnderssonLC. K562: A human leukemia cell line with erythroid features. Semin Hematol1981; 18: 72–7.
16.
McCoyJLHerbermanRBPerlinELevinePCrandallA. 51Cr release cellular lymphocyte cytotoxicity as a possible measure of immunological competence of cancer patients [Abstract]. Proc Am Assoc Cancer Res1973; 14: 107.
17.
GarottaGGiannobiPMasseriniCOttavianiFScaramelliniG. Attivita NK dei linfonodi locoregionali di pazienti affetti da neoplasie maligne della laringe. Comunicazione effettuata al 68° Congresso Soc Ital ORL, Grado 1981, Atti del Congresso.
18.
MenzioPCavalloGCortesinaG. Problemi immunologici nel cancro della laringe. Relazione Ufficiale 68° Congresso Soc Ital ORL, Grado 1981, Atti del Congresso.
19.
BoyumA. Isolation of mononuclear cells and granulocytes from human blood: Isolation of mononuclear cells by one centrifugation, and granulocytes by combining centrifugation and sedimentation at Ig. Scand J Clin Lab Invest1968; (suppl 97) 21: 77–85.
20.
BrunnerKHCerottiniJC. In vitro assay of target cell lysis by sensitized lymphocytes. In: BloomBRGladePR, eds. In vitro methods in cell-mediated and tumor immunity. New York: Academic Press, 1976: 369–73.
