In Vitro Cultivation of the Fetal Guinea Pig Inner Ear

Abstract

Fetal guinea pig inner ears, gestational age from 21 to 54 days, were cultivated in vitro and then observed with light and electron microscopes. Early 21-day gestation otocysts developed cartilaginous capsules from differentiating mesenchyme and discrete neurosensory structures from endolabyrinthine epithelium. Coiling of the cochlea occurred. Older, differentiated inner ears showed progressive increase in size and development of the labyrinth. The 26-day gestation cochlea, maintained for 12 days in vitro, doubled in size with development comparable to the 37-day gestation in vivo control. The large size of the guinea pig inner ear resulted in varying degrees of necrosis. However, the well-defined growth and development of the guinea pig fetal inner ear in vitro indicates the efficacy of the technique and suggests that similar success would be forthcoming with other animals used in audiologic research, such as chinchilla and hamster.

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References

Van de Water

Ruben

RJ.

Growth of the inner ear in organ culture. Ann Otol Rhinol Laryngol 1974; 83 Suppl 14: 1–16.

Van de Water

TR.

Effects of removal of the statoacoustic ganglion complex upon the growing otocyst. Ann Otol Rhinol Laryngol 1976: 85 Suppl 33: 2–32.

Friedmann

The ear. In: Willmer

Cells and tissues in culture: methods, biology and physiology. Vol 2. New York: Academic Press, 1965: 521–47.

Friedmann

Pathology of the ear. Oxford: Blackwell Scientific Publications, 1974.

Friedmann

Hodges

Riddle

PN.

Organ culture of the avian and mammalian otocyst. Acta Otolaryngol (Stockh) 1977; 83: 170–4.

Sobkowicz

Bereman

Rose

JE.

Organotypic development of the organ of Corti in culture. J Neurocytol 1975; 4: 543–72.

Yamashita

Vosteen

KH.

Tissue culture of the organ of Corti and the isolated hair cells from the newborn guinea pig. Acta Otolaryngol (Stockh) 1975; 30 Suppl 3: 77–90.

Anniko

Wersall

The inner ear and the in vitro system. Arch Otorhinolaryngol (NY) 1979; 224: 25–30.

Wagner

Manning

Biology of the guinea pig. New York: Academic Press, 1976.

10.

Matthews

Jackson

Pregnancy diagnosis on the guinea pig. Lab Anim Sci 1977; 27: 248–50.

11.

Draper

RL.

The prenatal growth of the guinea pig. Anat Rec 1920; 18: 369–92.

12.

Anniko

Van de Water

Nordemar

Organ culture of the 16th gestational day mouse labyrinth: a model suggestion for pre- and post-partum development. Acta Otolaryngol (Stockh) 1978; 86: 52–5.

13.

Chodynicki

Embryogenesis of the auditory part of the inner ear in the guinea pig. Acta Theriol 1968; 13: 219–60.

14.

Van de Water

Ruben

RJ.

The fate mapping of the eleventh and twelfth day mouse otocyst: an in vitro study of the sites or origin of the embryonic inner ear sensory structures. J Morphol 1978; 157: 249–67.

15.

Friedmann

Bird

ES.

The effect of ototoxic antibiotics and of penicillin on the sensory areas of the isolated fowl embryo otocyst in organ culture: an EM study. J Pathol Bacteriol 1961; 81: 81–90.

16.

Friedmann

The effect of sodium cyanide on the chick embryo otocyst in vitro: a tissue culture study. Acta Otolaryngol (Stockh) 1972; 73: 280–9.

17.

McPhee

Influences on the coiling of the cochlea. Ann Otol Rhinol Laryngol 1979; 88: 280–7.

18.

Saver

Van de Water

TR.

Morphogenesis and cytodifferentiation of the mouse otocyst following exposure to trypsin. Abstracts of the Third Midwinter Research Meeting of the Association for Research in Otolaryngology, abstract 3. St. Petersburg, Florida, January 21, 1980.

19.

Van de Water

Ruben

RJ.

III

Organ culture of the mammalian inner ear: a tool to study inner ear deafness. Laryngoscope 1974; 84: 738–49.

20.

Davis

Hawrisiak

MM.

Experimental cytomegalovirus infection and the developing mouse inner ear: in vivo and in vitro studies. Lab Invest 1977; 37: 20–9.

21.

Davis

Hawrisiak

MM.

Guinea pig cytomegalovirus infection of the guinea pig inner ear cultivated in vitro. Abstracts of the Third Midwinter Meeting of the Association for Research in Otolaryngology, abstract 30. St. Petersburg, Florida, January 23, 1980.