We succeeded in deriving a long-term tissue culture cell line from human maxillary sinus carcinoma. This cell line, designated as MC, was passaged 100 times in vitro over a period of 18 months. The cells are globular in shape, grow as single cells in the culture medium, and the mean population doubling time is about 12 hours. The plating efficiency rate in soft agar is 78% and chromosomal analysis revealed the modal chromosome number to be between 47 and 51. These MC cells were transplanted into five nude mice, all of which developed a tumor after a latency period of 5 to 8 days and died within 39 days. Complete autopsy of all mice revealed no metastasis. Histopathological findings of the original and the transplanted tumor tissues showed a remarkable similarity.
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References
1.
MooreAESabachewskyLToolanHW. Culture characteristics of four permanent lines of human cancer cells. Cancer Res1955; 15: 598–602.
2.
IshidaMNakajimaAInoueH. Successive culture of cancerous cells originating in the human paranasal sinus. Jpn J Otol (Tokyo)1976; 79: 686–94.
3.
KleinEKleinG. Antigenic properties of lymphomas induced by the moloney agent. J Natl Cancer Inst1964; 32: 547–68.
4.
KleinGCliffordPKleinE. Search for tumor-specific immune reactions in Burkitt lymphoma patients by the membrane immunofluorescence reaction. Proc Natl Acad Sci USA1966; 55: 1628–35.
5.
WybranJCarrMCFudenbergHH. The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest1972; 51: 2537–43.
6.
EasthamRJMasonJMJenningsBR. T-cell rosette test in squamous cell carcinoma of the head and neck. Arch Otolaryngol1976; 102: 171–5.
7.
KurokiT. Agar plate culture and Lederberg-style replica plating of mammalian cells. In: PrescottDM, ed. Methods in cell biology, vol. 9. New York: Academic Press, 1975: 158–78.
8.
MacPhersonIMontagnierL. Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology1964; 23: 291–4.
9.
GiovanellaBCYimSOStehlinJS. Development of invasive tumors in the “nude” mouse after injection of cultured human malanoma cells. J Natl Cancer Inst1972; 48: 1531–3.
10.
KugaNYoshidaKSeidoT. Heterotransplantation of cultured human cancer cells and human cancer tissues into nude mice. Gann1975; 66: 547–60.
11.
OzzelloLSordatBMerendaC. Transplantation of human mammary carcinoma cell line (BT20) into nude mice. J Natl Cancer Inst1974; 52: 1669–72.
12.
McConnellI. T and B lymphocytes. In: HobartMJMcConnellI, eds. The immune system. London: Blackwell Scientific Publication, 1976: 98–119.
13.
CoombsRRAGurnerBWWilsonAB. Rosette formation between human lymphocytes and sheep red cells not involving immunoglobulin receptors. Int Arch Allergy Appl Immunol1970; 39: 658–63.
14.
CarrelSSordatBMerendaC. Establishment of a cell line (Co-115) from a human colon carcinoma transplanted into nude mice. Cancer Res1976; 36: 3978–84.
15.
FoghJTrempeG. New human tumor cell lines. In: FoghJ, ed. Human tumor cell in vitro. New York: Plenum Press, 1975: 115–51.
16.
GeyGOGoffmanWDKubicerMT. The tissue culture study on the proliferative capacity of cervical carcinoma and normal epithelium. abstract. Cancer Res1952; 12: 264–5.
17.
McAllisterRMReedG. Colonial growth in agar of cells derived from neoplastic and non-neoplastic tissues of children. Pediatr Res1968; 2: 356–60.
GiardDJAaronsonSATodaroGJ. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J Natl Cancer Inst1973; 51: 1417–22.
20.
SandbergAAHossfeldDK. Chromosomal abnormalities in human neoplasia. Ann Rev Med1970; 21: 379–408.
21.
AtkinNBBakerMC. Chromosomal abnormalities as primary events in human malignant disease: evidence from marker chromosomes. J Natl Cancer Inst1966; 36: 539–57.
22.
GiovanellaBCStehlinJSWilliamsLJJr. Heterotransplantation of human malignant tumors in “nude” thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from human solid tumors. J Natl Cancer Inst1974; 52: 921–7.
23.
MedoffGKwanCNSchlessingerD. Potentiation of rifampicin, rifampicin analogues and tetracycline against animal cells by amphotericin B and polymixin B. Cancer Res1973; 33: 1146–9.
24.
KomiyamaSHirotoIKuwanoM. Enhancement of radiation effect on transformed fibroblastic cells by the synergistic combination of 5-fluorouracil and polyenes in vitro. Gann1974; 65: 85–7.
25.
NakashimaTKuwanoMMatsuiK. Potentiation of bleomycin by an antifungal polyene, pentamycin, in transformed animal cells. Cancer Res1974; 34: 3258–61.
26.
KomiyamaSHirotoIRyuS. Synergistic combination therapy of 5-fluorouracil, vitamin A and cobalt-60 radiation upon head and neck tumors. Oncology (in press).
