Experiments were conducted in the guinea pig cochlea and the Xenopus laevis lateral line organ to obtain information about the electrical impedance properties of the cochlea, the sources and characteristics of the cochlear potentials, and to determine if hair cells are electrically excitable. The significance of the resting and evoked cochlear potentials in the transduction process leading to excitation of the acoustic nerve fibers was evaluated by comparing the results of electrophysiological experiments with the prediction of a model of the cochlea designed after the mechanoelectric theory of hair cell function. The results of these experiments 1) were compatible with the prediction of the mechoelectric theory of cochlear function; 2) made it possible to estimate and compare the energy dissipated in generating and maintaining the cochlear potentials with the stores of biochemical energy available in the cochlea; and 3) showed that the hair cells are electrically excitable, from which it was concluded that interaction between the hair cells is feasible.
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References
1.
DavisH: Some principles of sensory receptor action. Physiol Rev41: 391–416, 1961.
2.
FuortesMGF: Generation of responses in a receptor, in LowensteinWR, (ed): Handbook of Sensory Physiology: I. New York: Springer-Verlag, 1971, pp 243–268.
3.
BaulcnightRSStrelioffDHonrubiaV: Effective stimulus for the Xenopus laevis lateral line hair cell system. Laryngoscope, to be published.
4.
LowensteinOWersällJ: A functional interpretation of the electron microscopic structure of the sensory hairs in the cristae of the elasmobranch Raja clavata in terms of directional sensitivity. Nature (Lond)184: 1807–1810, 1959.
5.
GoldbergJMFernandezC: Physiology of peripheral neurons innervating semicircular canals of the squirrel monkey. I. Resting discharge and response to constant angular accelerations. J Neurophysiol34: 635–660, 1971.
6.
HarrisGGFrishkopfFSFlockÅ: Receptor potentials from hair cells of the lateral line. Science167: 76–79, 1970.
7.
WeissTFMulroyMJAltmannDW: Intracellular responses to acoustic clicks in the inner ear of the alligator lizard. J Acoust Soc Am55: 606–619, 1974.
8.
DavisHDerbyshireAJLurieMH: The electric response of the cochlea. Am J Physiol107: 311–332, 1934.
9.
TasakiI: Nerve impulses in individual nerve fibers of guinea pig. J Neurophysiol17: 97–122, 1954.
10.
KianeNYSWatanabeTThomasEC: Discharge Patterns of Single Fibers in the Cat's Auditory Nerve. Res Monograph 35, Cambridge, MIT Press, 1965.
11.
RoseJEBruggeJFAndersonDJ: Phase-locked response to low-frequency tones in single auditory nerve fibers of the squirrel monkey. J Neurophysiol30: 769–793, 1967.
12.
FurukawaTIshiiY: Neurophysiological studies on hearing in gold fish. J Neurophysiol30: 1377–1403, 1967.
13.
FlockÅ: Sensory transduction in hair cells, in LowensteinWR, (ed): Handbook of Sensory Physiology: I. New York: Springer-Verlag, 1971, pp 396–441.
14.
SandOOzawaSHagiwaraS: Electrical and mechanical stimulation of hair cells in the mudpuppy. J Comp Physiol Psychol102: 13–26, 1975.
15.
DavisH: A model for transducer action in the cochlea. Cold Spring Harbor Symp Quant Biol30: 181–190, 1965.
16.
von BékésyG: Experiments in Hearing. New York: McGraw-Hill, 1960.
17.
de NóR Lorente: The sensory endings in the cochlea. Laryngoscope47: 373–377, 1937.
18.
KimuraRS: The ultrastructure of the organ of Corti. Int Rev Cytol42: 173–222, 1975.
19.
SpoendlinH: The innervation of the cochlear receptor, in MøllerA, (ed): Basic Mechanisms in Hearing. New York: Academic Press, 1973, pp 185–234.
20.
TasakiISpyropoulosCS: Stria vascularis as source of endocochlear potential. J Neurophysiol22: 149–155, 1959.
21.
DavisHDeatherageBHRosenblutB: Modification of cochlear potentials produced by streptomycin poisoning and by extensive venous obstruction. Laryngoscope68: 596–627, 1958.
22.
HonrubiaVWardPH: Dependence of the cochlear microphonics and summating potential on the endocochlear potential. J Acoust Soc Am46: 338–392, 1969.
23.
StrelioffDHaasGHonrubiaS: Sound-induced electrical impedance changes in the guinea pig cochlea. J Acoust Soc Am51: 617–620, 1972.
24.
HonrubiaVStrelioffDWardPH: A quantitative study of cochlear potentials along the scala media of the guinea pig. J Acoust Soc Am54: 600–609, 1973.
25.
StrelioffDHonrubiaVBauknightRS: Stimulus control for the study of the Xeopus laevis lateral line hair cell system. J Electro-physiol Tech5: 35–43, 1976.
26.
HonrubiaVWardPH: Mechanism of production of cochlear microphonics. J Acoust Soc Am47: 498–503, 1970.
27.
StrelioffD: A computer simulation of the generation and distribution of cochlear potentials. J Acoust Soc Am54: 620–628, 1973.
28.
ButlerRA: Some experimental observations on the dc resting potentials in the guinea pig cochlea. J Acoust Soc Am37: 429–433, 1965.
29.
KuijpersWBontingSL: The cochlear potentials: II. The nature of the cochlear resting potentials. Pfluegers Arch320: 359–372, 1970.
30.
HonrubiaVJohnstoneBMButlerRA: Maintenance of cochlear potentials during asphyxia. Acta Otolaryngol (Stockh)60: 105–112, 1965.
31.
JohnstoneBM: Genesis of the cochlear endolymphatic potential, in SanadiDR, (ed): Current Topics in Bioenergetics. New York: Academic Press, 1967, pp 335–352.
32.
PrazmaJThomasWGFisherND: Ototoxicity of ethacrynic acid. Arch Otolaryngol95: 448–456, 1972.
33.
BosherSKSmithCWarrenRL: The effects of ethacrynic acid upon the cochlear endolymph and stria vascularis. Acta Otolaryngol75: 184–191, 1973.
34.
ThalmannRKusakariJMiyoshiT: Dysfunction of energy releasing and consuming processes of the cochlea. Laryngoscope83: 1690–1712, 1973.
35.
KonishiTKelseyESingletonGT: Negative potentials in scala media during early stages of anoxia. Acta Otolaryngol (Stockh)64: 107–118, 1967.
36.
GeislerCDRhodeWSKennedyDT: Responses to tonal stimuli of single auditory nerve fibers and their relationship to basilar membrane motion in the squirrel monkey. J Neurophysiol37: 1156–1172, 1974.
37.
RobertsonD: Cochlear neurons: Frequency selectivity altered by perilymph removal. Science186: 153–155, 1974.
38.
LowryOHPassonneauJVHassel-BergerFX: Effect of ischemia on known substrates and cofactors of the glycolytic pathway in the brain. J Biol Chem239: 18–20, 1964.
39.
HonrubiaVStrelioffDSitkoST: Electroanatomy of the cochlea: Its role in cochlear potential measurements, in RubenRJElberlingCSalamonG, (eds): Electrococh-leography. Baltimore, University Park Press, to be published.
40.
GeislerCDAdrianHOMountainDC: Electrical impedance changes in the guinea pig cochlea recorded during tonal stimuli. J Acoust Soc Am58, Suppl No. 1: S31, 1975.
41.
KarlanMSTonndorfJKhannaSM: Dual origin of the cochlear microphonics, inner and outer hair cells. Ann Otol Rhinol Laryngol81: 696–704, 1972.
42.
DallosP: Cochlear potentials and cochlear mechanics, in MøllerA, (ed): Basic Mechanisms of Hearing. New York: Academic Press, 1973, pp 335–372.
43.
KiangNYSMoxonECLevineRA: Auditory-nerve activity in cats with normal and abnormal cochleas, in WolstenholmeGEWKnightJ, (eds): Sensorineural Hearing Loss. London: Churchill, 1970, pp 241–268.
44.
RyanADallosP: Effect of absence of cochlear outer hair cells on behavioural auditory threshold. Nature253: 44–46, 1975.
45.
ZwislockiJJSokolichWG: Neuro-mechanical frequency analysis in the cochlea, in ZwickerETerhardtE, (eds): Facts and Models in Hearing. New York: Springer- Verlag, 1974, pp 107–177.
46.
JohnstoneJRJohnstoneBMYatesGK: Cochlear neurons: Frequency selectivity altered by conductance change in scala tympani. J Acoust Soc Am57: 1210–1211, 1975.
47.
StrelioffDSitkoSTHonrubiaV: Role of inner and outer hair cells in neural excitation. Trans Am Acad Ophthalmol Otolaryngol, to be published.
