Changes in Permeability of Strial Vessels following Vibration Given to Auditory Ossicle by Drill

Abstract

We produced drill-induced damage of the auditory ossicles of guinea pigs to study changes over time in the permeability of the blood vessels of the stria vascularis to horseradish peroxidase (HRP). In group A, the stimulus was applied for 10 seconds after intravenous injection of HRP In group B, it was applied for 30 seconds, and in group C, for 60 seconds. The cochlea was fixed with 2% glutaraldehyde perfused through the round window, and the guinea pigs were then decapitated. The stria vascularis of the basal and third turns was examined. The leakage of HRP from the blood vessels of the stria vascularis significantly increased in relation to the duration of the stimulus in both the basal and third turns. The damage to intermediate cells also tended to be in relation to the duration of the stimulus. Extravascular permeation of HRP took place through the tubules in the endothelial cytoplasm. The vibratory stimulation presumably opened channels that are not normally open.

Keywords

drilling trauma horseradish peroxidase stria vascularis

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References

Palva

Karja

Palva

. High-tone sensorineural losses following chronic ear surgery. Arch Otolaryngol 1978; 98: 176–8.

Smyth

GDL

. Sensorineural hearing loss in chronic ear surgery. Ann Otol Rhinol Laryngol 1977; 86: 3–8.

Tos

Lau

Plate

. Sensorineural hearing loss following chronic ear surgery. Ann Otol Rhinol Laryngol 1984; 93: 403–9.

Paparella

. Acoustic trauma from the bone cutting bur. Laryngoscope 1962; 72: 116–26.

Gjuric

Schneider

Wolf

Wigand

. Experimental sensorineural hearing loss following drill-induced ossicular chain injury. Acta Otolaryngol (Stockh) 1997; 117: 497–500.

Watanabe

Komatsuzaki

. Permeability for horseradish peroxidase in strial capillaries in each turn of cochlea. ORL J Otorhinolaryngol Relat Spec 1994; 56: 183–9.

Schuknecht

Tonndorf

. Acoustic trauma of the cochlea from ear surgery. Laryngoscope 1960; 70: 479–505.

Urquhart

McIntosch

Bodenstein

. Drill-generated sensorineural hearing loss following mastoid surgery. Laryngoscope 1992; 102: 689–92.

Domenech

Carulla

Traserra

. Sensorineural high-frequency hearing loss after drill-generated acoustic trauma in tympanoplasty. Arch Otorhinolaryngol 1989; 246: 280–2.

10.

Hegewald

Heitman

Wiederhold

Cooper

Gates

. High-frequency electrostimulation hearing after mastoidectomy. Otolaryngol Head Neck Surg 1989; 100: 49–56.

11.

Hüttenbrink

. Die Cochlea schädigung durch mittelohr-operationen. Laryngorhinootologie 1991; 70: 66–71.

12.

Kylen

Arlinger

. Drill-generated noise levels in ear surgery. Acta Otolaryngol (Stockh) 1976; 82: 402–29.

13.

Suits

Brummett

Nunley

. Effect of otologic drill noise on ABR thresholds in a guinea pig model. Otolaryngol Head Neck Surg 1993; 109: 660–7.

14.

Graham

Kamovsky

. The early stage of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney. J Histochem Cytochem 1966; 14: 291–302.

15.

Duvall

Quick

Sutherland

. Horseradish peroxidase in the lateral cochlea wall. Arch Otolaryngol 1971; 93: 304–16.

16.

Winther

. The permeability of the guinea pig cochlea capillaries to horseradish peroxidase. Z Zellforsch 1971; 114: 193–202.

17.

Watanabe

. Change in the capillary permeability of the stria vascularis by different methods of death and fixation. Ann Otol Rhinol Laryngol 1986; 95: 427–31.

18.

Watanabe

Tanaka

. Horseradish peroxidase permeation from the capillaries of the stria vascularis after inoculation of endotoxin into the middle ear. Ann Otol Rhinol Laryngol 1997; 106: 394–8.