Abstract
The purpose of the present study was to evaluate the acoustic properties of myocytes in normal, pressure-overload hypertrophic, and amyloid myocardium. Myocardial tissue specimens at autopsy were obtained from 10 subjects without cardiovascular disease, six patients with left ventricular (LV) hypertrophy, and six patients with cardiac amyloi dosis. Sound speed of myocytes was measured at subendocardial and subepicardial regions in myocardium by use of a high-frequency (450 MHz) acoustic microscope. In normal myocardium, the sound speed of myocytes was significantly higher in subendo cardial region (1,728 ± 19 m/sec) than in subepicardial region (1,645 ±22 m/sec) (p < 0.0001). A significantly higher sound speed of myocytes was observed in the suben docardial region in LV hypertrophic myocardium (1,779 ± 19 m/sec) than that in normal myocardium (p < 0.001). In amyloid myocardium, a significantly lower sound speed of myocytes was observed in subendocardial (1,560 ± 8 m/sec) and subepicardial (1,594 ± 48 m/sec) regions than that in respective regions of the normal myocardium (p < 0.0001 and p < 0.05, respectively). Transmural variation in sound speed of myocytes measured by high-frequency acoustic microscopy existed in normal left ventricle. The differential myocyte sound speed and its transmural variation was observed in LV hyper trophic and amyloid myocardium as compared with normal myocardium. High-frequency acoustic microscopy can be a promising technique for myocardial tissue characterization at the myocyte level.
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