Acupuncture has been associated with improved cerebral circulation, analgesia, neuromodulatory function and neurogenesis. In particular, acupuncture at ST36 has been widely used in several central nervous system (CNS) disorders, including neurodegenerative diseases. However, its effects on hydrocephalus have not been studied. Our aim was to evaluate the effects of acupuncture at ST36 on behaviour, motor development and reactive astrogliosis in infantile rats with hydrocephalus.
Methods
Hydrocephalus was induced in sixteen 7-day-old pup rats by injection of 20% kaolin into the cisterna magna. One day after hydrocephalus induction, acupuncture was applied once daily (for 30 min) for a total of 21 days in eight randomly selected animals (HAc group) while the remaining eight remained untreated (H group). An additional eight healthy animals were included as controls (C group). All animals were weighed daily and, from the fifth day after hydrocephalus induction, underwent MRI to determine the ventricular ratio (VR). Rats were also exposed to modified open-field tests every 3 days until the end of the experiment. After 21 days all the animals were euthanased and their brains removed for histology and immunohistochemistry.
Results
Hydrocephalic rats showed an increase in VR when compared with control rats (P<0.01). In addition, these animals exhibited delayed weight gain, which was attenuated with acupuncture treatment. Hydrocephalic animals treated with acupuncture performed better in open field tests (P<0.05), and had a reduction in reactive astrocyte cell density in the corpus callosum and external capsule, as assessed by GFAP (glial fibrillary acidic protein) immunohistochemistry (P<0.05).
Conclusions
These findings indicate that acupuncture at ST36 has a neuroprotective potential mediated, in part, by inhibition of astrogliosis.
Del BigioMR. Neuropathological changes caused by hydrocephalus. Acta Neuropathol1993;85:573–85. doi:10.1007/BF00334666
2.
Del BigioMR, CrookCR, BuistR.Magnetic resonance imaging and behavioral analysis of immature rats with kaolin-induced hydrocephalus: pre- and postshunting observations. Exp Neurol1997;148:256–64. doi:10.1006/exnr.1997.6644
JonesHC, RichardsHK, BucknallRM, Local cerebral blood flow in rats with congenital hydrocephalus. J Cereb Blood Flow Metab1993;13:531–4. doi:10.1038/jcbfm.1993.69
5.
Del BigioMR, ZhangYW. Cell death, axonal damage, and cell birth in the immature rat brain following induction of hydrocephalus. Exp Neurol1998;154:157–69. doi:10.1006/exnr.1998.6922
6.
ChoZH, ChungSC, JonesJP, New findings of the correlation between acupoints and corresponding brain cortices using functional MRI. Proc Natl Acad Sci U S A1998;95:2670–3. doi:10.1073/pnas.95.5.2670
7.
KimJH, MinBI, SchmidtD, The difference between electroacupuncture only and electroacupuncture with manipulation on analgesia in rats. Neurosci Lett2000;279:149–52. doi:10.1016/S0304-3940(99)00994-5
8.
WuP, MillsE, MoherD, Acupuncture in poststroke rehabilitation: a systematic review and meta-analysis of randomized trials. Stroke2010;41:e171–9. doi:10.1161/STROKEAHA.109.573576
9.
TaoJ, ZhengY, LiuW, Electro-acupuncture at LI11 and ST36 acupoints exerts neuroprotective effects via reactive astrocyte proliferation after ischemia and reperfusion injury in rats. Brain Res Bull2016;120:14–24. doi:10.1016/j.brainresbull.2015.10.011
10.
ZengBY, ZhaoK.Effect of acupuncture on the motor and nonmotor symptoms in Parkinson's disease–a review of clinical studies. CNS Neurosci Ther2016;22:333–41. doi:10.1111/cns.12507
11.
ZhouY, JinJ.Effect of acupuncture given at the HT 7, ST 36, ST 40 and KI 3 acupoints on various parts of the brains of Alzheimer' s disease patients. Acupunct Electrother Res2008;33:9–17. doi:10.3727/036012908803861186
12.
LiaoET, TangNY, LinYW, Long-term electrical stimulation at ear and electro-acupuncture at ST36-ST37 attenuated COX-2 in the CA1 of hippocampus in kainic acid-induced epileptic seizure rats. Sci Rep2017;7:472. doi:10.1038/s41598-017-00601-1
13.
LuL, ZhangXG, ZhongLL, Acupuncture for neurogenesis in experimental ischemic stroke: a systematic review and meta-analysis. Sci Rep2016;6:19521. doi:10.1038/srep19521
14.
ChenY, ZhouJ, LiJ, Electroacupuncture pretreatment prevents cognitive impairment induced by limb ischemia-reperfusion via inhibition of microglial activation and attenuation of oxidative stress in rats. Brain Res2012;1432:36–45. doi:10.1016/j.brainres.2011.11.002
15.
KhanOH, EnnoTL, Del BigioMR. Brain damage in neonatal rats following kaolin induction of hydrocephalus. Exp Neurol2006;200:311–20. doi:10.1016/j.expneurol.2006.02.113
16.
McAllisterJP, ChovanP.Neonatal hydrocephalus. Mechanisms and consequences. Neurosurg Clin N Am1998;9:73–93.
17.
DaviesSJ, GoucherDR, DollerC, Robust regeneration of adult sensory axons in degenerating white matter of the adult rat spinal cord. J Neurosci1999;19:5810–22.
18.
LopesLS, SlobodianI, Del BigioMR. Characterization of juvenile and young adult mice following induction of hydrocephalus with kaolin. Exp Neurol2009;219:187–96. doi:10.1016/j.expneurol.2009.05.015
19.
Rocha CatalãoCH, Leme CorreaDA, Bernardino GarciaCA, Pre- and postshunting magnetization transfer ratios are in accordance with neurological and behavioral changes in hydrocephalic immature rats. Dev Neurosci2014;36:520–31. doi:10.1159/000366056
20.
CatalãoCHR, ShimizuGY, TidaJA, Environmental enrichment reduces brain damage in hydrocephalic immature rats. Childs Nerv Syst2017;33:921–31. doi:10.1007/s00381-017-3403-4
21.
ZhangX, YuanY, KuangP, Effects of electro-acupuncture on somatostatin and pancreatic polypeptide in ischemic cerebrovascular diseases. J Tradit Chin Med1999;19:54–8.
22.
Del BigioMR, WilsonMJ, EnnoT.Chronic hydrocephalus in rats and humans: white matter loss and behavior changes. Ann Neurol2003;53:337–46. doi:10.1002/ana.10453
23.
OlopadeFE, ShokunbiMT, SirénAL. The relationship between ventricular dilatation, neuropathological and neurobehavioural changes in hydrocephalic rats. Fluids Barriers CNS2012;9:19. doi:10.1186/2045-8118-9-19
24.
Del BigioMR, KanferJN, ZhangYW. Myelination delay in the cerebral white matter of immature rats with kaolin-induced hydrocephalus is reversible. J Neuropathol Exp Neurol1997;56:1053–66. doi:10.1097/00005072-199709000-00010
25.
KimEH, JangMH, ShinMC, Acupuncture increases cell proliferation and neuropeptide Y expression in dentate gyrus of streptozotocin-induced diabetic rats. Neurosci Lett2002;327:33–6. doi:10.1016/S0304-3940(02)00372-5
26.
KimEH, KimYJ, LeeHJ, Acupuncture increases cell proliferation in dentate gyrus after transient global ischemia in gerbils. Neurosci Lett2001;297:21–4. doi:10.1016/S0304-3940(00)01656-6
27.
PeknyM, WilhelmssonU, BogestålYR, The role of astrocytes and complement system in neural plasticity. Int Rev Neurobiol2007;82:95–111. doi:10.1016/S0074-7742(07)82005-8
28.
FukumizuM, TakashimaS, BeckerLE. Glial reaction in periventricular areas of the brainstem in fetal and neonatal posthemorrhagic hydrocephalus and congenital hydrocephalus. Brain Dev1996;18:40–5. doi:10.1016/0387-7604(95)00103-4
29.
HuangL, WuZB, ZhugeQ, Glial scar formation occurs in the human brain after ischemic stroke. Int J Med Sci2014;11:344–8. doi:10.7150/ijms.8140
30.
PeknyM, WilhelmssonU, PeknaM.The dual role of astrocyte activation and reactive gliosis. Neurosci Lett2014;565:30–8. doi:10.1016/j.neulet.2013.12.071
31.
GonzálezH, ElguetaD, MontoyaA, Neuroimmune regulation of microglial activity involved in neuroinflammation and neurodegenerative diseases. J Neuroimmunol2014;274:1–13. doi:10.1016/j.jneuroim.2014.07.012
32.
FarinaC, AloisiF, MeinlE.Astrocytes are active players in cerebral innate immunity. Trends Immunol2007;28:138–45. doi:10.1016/j.it.2007.01.005
33.
TangWC, HsuYC, WangCC, Early electroacupuncture treatment ameliorates neuroinflammation in rats with traumatic brain injury. BMC Complement Altern Med2016;16:470. doi:10.1186/s12906-016-1457-6
34.
LiF, YanCQ, LinLT, Acupuncture attenuates cognitive deficits and increases pyramidal neuron number in hippocampal CA1 area of vascular dementia rats. BMC Complement Altern Med2015;15:133. doi:10.1186/s12906-015-0656-x
35.
MaR, YuanB, DuJ, Electroacupuncture alleviates nerve injury after cerebra ischemia in rats through inhibiting cell apoptosis and changing the balance of MMP-9/TIMP-1 expression. Neurosci Lett2016;633:158–64. doi:10.1016/j.neulet.2016.09.033
36.
HsuJY, BourguignonLY, AdamsCM, Matrix metalloproteinase-9 facilitates glial scar formation in the injured spinal cord. J Neurosci2008;28:13467–77. doi:10.1523/JNEUROSCI.2287-08.2008
37.
LinR, YuK, LiX, Electroacupuncture ameliorates post-stroke learning and memory through minimizing ultrastructural brain damage and inhibiting the expression of MMP-2 and MMP-9 in cerebral ischemia-reperfusion injured rats. Mol Med Rep2016;14:225–33. doi:10.3892/mmr.2016.5227
