Physicochemical Properties of Basic Fibroblast Growth Factor-Gelatin Complex

Abstract

Change in physicochemical properties of basic fibroblast growth factor (bFGF) accompanied with gelatin mixing was studied by agarose gel electrophoresis, electrophoretic light scattering (ELS) and dynamic light scattering (DLS) experiments. When bFGF was mixed with the acidic gelatin with an isoelectric point (IEP) of 5.0 in aqueous solution, the electrophoretic mobility of bFGF to the negative electrode decreased with time, indicating reduced positive charge of bFGF. The bFGF mobility became lower with an increase in the ratio of the acidic gelatin to bFGF, in contrast to the basic gelatin with an IEP of 9.0. Upon mixing with heparin, a similar change in the electrophoretic mobility of bFGF was observed although the rate and extent were greater than that with bFGF-acidic gelatin. This is due to stronger electrostatic interaction between bFGF and heparin. ELS experiments revealed that the net charge of bFGF (+ 8.60 mV) was neutralized immediately after mixing with the acidic gelatin with a net charge of -6.79 mV, followed by no further value change. In DLS experiments the apparent molecular size of the acidic gelatin increased with time after bFGF mixing. It is possible that the acidic gelatin electrostatically interacted with bFGF to form a polyion complex, followed by the complex aggregation, resulting in the increased apparent molecular size.

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