Hydrolysis of Chitosan,Chitosan-Polyoxyethylene and Chitosan-Poly(2-acryloylamido-2-methylpropanesulfonic acid) by a Crude Enzyme Complex from Trichoderma viride

Abstract

The degradation of chitosan using a crude enzyme complex from the soil fungus Trichoderma viride was examined in terms of temperature, pH, enzyme activity and the presence of a polymer partner. It was found that chitosan hydrolysis is not suppressed by combining it with polyoxyethylene or poly(2-acryloylamido-2-methylpropanesulfonic acid). The results on Trichoderma viride immobilized on beads imply that chitosan, as well as its polymer complexes are appropriate carriers for development of ecologically safe phytosanitary preparations.

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References

1. Kombrink, E. and Somssich, I.E. (1995). Adv. Bot. Res., 21: 2-2.

2. Stoyachenko, I.A. , Varlamov, V.P. and Davankov, V.A. (1994). Carbohyd. Polym., 24: 47-47.

3. Harman, G.E. , Hayes, C.K. , Lorito, M. , Broadway, R.M. , Di Pietro, A. , Peterbauer, C. and Tronsmo, A. (1993). Phytopathology, 83: 313-313.

4. Lorito, M. , Harman, G.E. , Hayes, C.K. , Broadway, R.M. , Tronsmo, A. , Woo, S.L. and Di Pietro, A. (1993). Phytopathology, 83: 302-302.

5. Manolova, N. et al., unpublished results.

6. Stoilova, O. , Koseva, N. , Manolova, N. and Rashkov, I. (1999). Polym. Bull., 43: 67-67.

7. Miller, G.M. (1959). Anal. Chem., 31: 426-426.

8. Chigaleichik, A.G. , Pirieva, D.A. and Rylkin, S.S. (1976). Prykl. Biokhim. Mikrobiol., (Russ.), 12: 581-581.

9. Koseva, N. , Stoilova, O. , Manolova, N. , Rashkov, I. and Madec, P.-J. (2001). J. Bioactive Compatible Polym., 16: 3-3.

10.

10. Muraki, E. , Yaku, F. and Kojima, H. (1993). Carbohydr. Res., 239: 227-227.

11.

11. Gamzazade, A.I. , Shlimac, V.M. , SkIjar, A.M. , Stykova, E.V. , Pavlova, S.A. and Rogozin, S.V. (1985). Acta Polymerica, 36: 421-421.

12.

12. Nikolova, A. , Manolova, N. and Rashkov, I. (1998). Polymer Bull., 41: 115-115.