A process to separate chitosan from Penicillium chrysogenum mycelium was developed. Alkaline treatment and acetic acid extraction were used for deacetylating chitin to chitosan. The molecular weight of the chitosan was 3.6 × 104 and the degree of deacetylation was 84%. Analysis indicated that the chitosan from Penicillium chrysogenum mycelium was similar to that obtained from shrimp. The chitosan from the mycelium was used to prepare microcapsules for controlled drug release. The chitosan microcapsules reached maximum release of vitamin D2 after 10 h which avoided the problem of instability in gastric juice; the chitosan microcapsules are suitable for adsorption of vitamin D2 in intestines.
2. Davoust, N. and Persson, A. (1992). Appl. Microbiol. Biotechnol., 37: 572–575.
3.
3. Okmi, P. (1992). Euro Pat Application, 0531991A2.
4.
4. Rane, K.D. and Hoove, D.G. (1993). Process Biochemistry, 28: 115–121.
5.
5. Crestini, C., Kovac, B. and Giovannozz-Sermanni, G. (1996). Biotechnology and Bioengineering, 50: 207–210.
6.
6. Knorr, D. (1991). Food Technology, 1: 114–118.
7.
7. He, X.J., Zhang, B.L. and Tan, T.W. et al. (2001). J. Chem. Eng. of Chinese University, 15(1): 23–28.
8.
8. Li, W., Tong, A. and Deng, Zh.Y. (1994). Chinese Journal of Biomedicine, 15(2): 96–99.
9.
9. Ma, B.X. (1974). Journal of Analytical Chemistry, 2(2): 53–56.
10.
10. Luo, Z.M. and Ceng, P.R. (1989). Chinese Journal of Physical and Chemical Measurement, 25(5): 274–276.
11.
11. National Environmental Protection Office (1989). Analytical Methods for Water Detection, China Environmental Science and Technology Press.
12.
12. Shi, X.Y. and Tan, T.W. (2002). Preparation of Chitosan/Ethylcellulose Complex Microcapsules and its Application in Controlled Release of Vitamin D2, Drug Biomaterials, 23-23 (accepted).
