Even though many experimental approaches benefit from tracking individual juvenile animals, there is yet to be a commercial zebrafish rack system designed to accomplish this task. Thus, we invented playpens, an acrylic, and screen container, to raise 12 individual zebrafish juveniles per standard 10 L tank on an existing recirculating fish system. During a week-long experiment, fish raised in playpens grow to the same size as conventionally raised juveniles.
Get full access to this article
View all access options for this article.
References
1.
FraherD, SanigorskiA, MellettNA, et al.Zebrafish embryonic lipidomic analysis reveals that the yolk cell is metabolically active in processing lipid. Cell Rep, 2016; 14(6):1317–1329; doi: 10.1016/j.celrep.2016.01.016
2.
WesterfieldM.The zebrafish book: A guide for the laboratory use of zebrafish (Danio rerio). Printed by the University of Oregon Press: Distributed by the Zebrafish International Resource Center Eugene, OR: Eugene, OR; 2007.
3.
LinboTL. Zebrafish (Danio rerio) Husbandry and Colony Maintenance at the Northwest Fisheries Science Center. U.S. Dept. Commer., NOAA Tech. Memo. NMFS-NWFSC-100, p. 62; 2009.
FariasM, CertalAC. Different feeds and feeding regimens have an impact on zebrafish larval rearing and breeding performance. Int J Marine Biol Res, 2016; 1(1):1–8.
6.
GuoY, LoganHL, GlueckDH, et al.Selecting a sample size for studies with repeated measures. BMC Med Res Methodol, 2013; 13:100; doi: 10.1186/1471-2288-13-100
7.
Siccardi IIIAJ, GarrisHW, JonesWT, et al.Growth and survival of zebrafish (Danio rerio) fed different commercial and laboratory diets. Zebrafish, 2009; 6(3):275–280.
8.
CartenJD, FarberSA. A new model system swims into focus: Using the zebrafish to visualize intestinal metabolism in vivo. Clin Lipidol, 2009; 4(4):501–515; doi: 10.2217/clp.09.40
9.
FarberSA, PackM, HoS-Y, et al.Genetic analysis of digestive physiology using fluorescent phospholipid reporters. Science, 2001; 292(5520):1385–1388.
10.
ThiererJH, EkkerSC, FarberSA. The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Nat Commun, 2019; 10(1):3426; doi: 10.1038/s41467-019-11259-w
11.
WilsonMH, EkkerSC, FarberSA. Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish. eLife, 2021; 10:e66393; doi: 10.7554/eLife.66393
