Abstract
Reactive oxygen species (ROS) are important regulators of intracellular signaling pathways in health and disease. It is implicated that ROS may play critical roles in pathogenesis of a number of kidney diseases including diabetic nephropathy. However, due to the lack of tools for in vivo detection of redox status, our knowledge of redox dynamics is still fragmentary. In this study, we present novel zebrafish UAS transgenic lines expressing mitochondrial and cytoplasmic targeted redox fluorescent biosensors, Grx1-roGFP2 and mitoGrx1-roGFP2. As the zebrafish is an ideal animal model for intravital imaging, these transgenic zebrafish provide useful tools to analyze renal redox dynamics in vivo.
Get full access to this article
View all access options for this article.
References
Supplementary Material
Please find the following supplemental material available below.
For Open Access articles published under a Creative Commons License, all supplemental material carries the same license as the article it is associated with.
For non-Open Access articles published, all supplemental material carries a non-exclusive license, and permission requests for re-use of supplemental material or any part of supplemental material shall be sent directly to the copyright owner as specified in the copyright notice associated with the article.
