In this study, we show that an adaptation of the spinning test can be used as a model to study the exercise–exhaustion–recovery paradigm in fish. This forced swimming test promotes a wide range of changes in the hypothalamus–pituitary–interrenal axis functioning, intermediary metabolism, as well in fish behavior at both exercise and recovery periods. Our results pointed that this adapted spinning test can be considered a valuable tool for evaluating drugs and contaminant effects on exercised fish. This can be a suitable protocol both to environmental—to evaluate contaminants that act in fish energy mobilization and recovery after stressors—and translational perspectives—effects of drugs on exercised or stressed humans.
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