For influenza hemagglutination inhibition (HAI) assays, species selection of red blood cells (RBCs) is critical to determine antibody titers to influenza viruses reliably. We compared pandemic influenza virus A/H1N1 (pdH1N1) HAI titers using turkey or guinea pig RBCs. Turkey RBCs appear to be the more appropriate species choice for influenza A/pH1N1 HAI assays.
Get full access to this article
View all access options for this article.
References
1.
BarrIG, McCauleyJ, CoxN, et al.Epidemiological, antigenic and genetic characteristics of seasonal influenza A(H1N1), A(H3N2) and B influenza viruses: basis for the WHO recommendation on the composition of influenza vaccines for use in the 2009–2010 Northern Hemisphere season. Vaccine, 2010; 28:1156–1167.
2.
BaumLG, and PaulsonJC. Sialyloligosaccharides of the respiratory epithelium in the selection of human influenza virus receptor specificity. Acta Histochem Suppl, 1990; 40:35–38.
3.
BrydakLB, and MachalaM. Humoral immune response to influenza vaccination in patients from high risk groups. Drugs, 2000; 60:35–53.
4.
ConnorRJ, KawaokaY, WebsterRG, and PaulsonJC. Receptor specificity in human, avian, and equine H2 and H3 influenza virus isolates. Virology, 1994; 205:17–23.
5.
CouceiroJN, PaulsonJC, and BaumLG. Influenza virus strains selectively recognize sialyloligosaccharides on human respiratory epithelium; the role of the host cell in selection of hemagglutinin receptor specificity. Virus Res, 1993; 29:155–165.
6.
CoudevilleL, BailleuxF, RicheB, MegasF, AndreP, and EcochardR. Relationship between haemagglutination-inhibiting antibody titres and clinical protection against influenza: development and application of a bayesian random-effects model. BMC Med Res Methodol, 2010; 10:18.
7.
de JongJC, PalacheAM, BeyerWE, RimmelzwaanGF, BoonAC, and OsterhausAD. Haemagglutination-inhibiting antibody to influenza virus. Dev Biol (Basel), 2003; 115:63–73.
8.
DucatezMF, CaiZ, PeirisM, et al.Extent of antigenic cross-reactivity among highly pathogenic H5N1 influenza viruses. J Clin Microbiol, 2011; 49:3531–3536.
9.
ImaiM, and KawaokaY. The role of receptor binding specificity in interspecies transmission of influenza viruses. Curr Opin Virol, 2012; 2:160–167.
10.
ItoT, SuzukiY, MitnaulL, VinesA, KidaH, and KawaokaY. Receptor specificity of influenza A viruses correlates with the agglutination of erythrocytes from different animal species. Virology, 1997; 227:493–499.
11.
KatzJM, HancockK, and XuX. Serologic assays for influenza surveillance, diagnosis and vaccine evaluation. Expert Rev Anti Infect Ther, 2011; 9:669–683.
12.
LowenAC, SteelJ, MubarekaS, CarneroE, Garcia-SastreA, and PaleseP. Blocking interhost transmission of influenza virus by vaccination in the guinea pig model. J Virol, 2009; 83:2803–2818.
13.
MakkochJ, PrachayangprechaS, PayungpornS, et al.Erythrocyte binding preference of human pandemic influenza virus a and its effect on antibody response detection. Ann Lab Med, 2012; 32:276–282.
14.
NamJH, KimEH, SongD, ChoiYK, KimJK, and PooH. Emergence of mammalian species-infectious and -pathogenic avian influenza H6N5 virus with no evidence of adaptation. J Virol, 2011; 85:13271–13277.
15.
PaulsonJC, and RogersGN. Resialylated erythrocytes for assessment of the specificity of sialyloligosaccharide binding proteins. Methods Enzymol, 1987; 138:162–168.
16.
RogersGN, and PaulsonJC. Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin. Virology, 1983; 127:361–373.
17.
RoweT, AbernathyRA, Hu-PrimmerJ, et al.Detection of antibody to avian influenza A (H5N1) virus in human serum by using a combination of serologic assays. J Clin Microbiol, 1999; 37:937–943.
18.
Ryan-PoirierKA, and KawaokaY. Distinct glycoprotein inhibitors of influenza A virus in different animal sera. J Virol, 1991; 65:389–395.
19.
SkehelJJ, and WileyDC. Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annu Rev Biochem, 2000; 69:531–569.
20.
StephensonI, WoodJM, NicholsonKG, and ZambonMC. Sialic acid receptor specificity on erythrocytes affects detection of antibody to avian influenza haemagglutinin. J Med Virol, 2003; 70:391–398.
21.
ThompsonCI, BarclayWS, and ZambonMC. Changes in in vitro susceptibility of influenza A H3N2 viruses to a neuraminidase inhibitor drug during evolution in the human host. J Antimicrob Chemother, 2004; 53:759–765.
22.
WangS, TaaffeJ, ParkerC, et al.Hemagglutinin (HA) proteins from H1 and H3 serotypes of influenza A viruses require different antigen designs for the induction of optimal protective antibody responses as studied by codon-optimized HA DNA vaccines. J Virol, 2006; 80:11628–11637.
23.
WeisW, BrownJH, CusackS, PaulsonJC, SkehelJJ, and WileyDC. Structure of the influenza virus haemagglutinin complexed with its receptor, sialic acid. Nature, 1988; 333:426–431.
24.
World Health Organization. 2002. WHO Manual on Animal Influenza Diagnosis and Surveillance, pp. 1–99.
25.
World Health Organization. 2009. Influenza (Seasonal). www.who.int/mediacentre/factsheets/fs211/en/. Accessed March20, 2013.
26.
World Health Organization. 2011. WHO Global Influenza Surveillance Network: Manual for the Laboratory Diagnosis and Virological Surveillance of Influenza, pp. 1–139.