As one of the important tick-borne zoonotic pathogens, Anaplasma has both veterinary and public health significance. Here, we performed a survey of Anaplasma infection in the goats from a farm in Beijing, China, and found 44.6% (41/92) were infected with Anaplasma capra, and 22.8% (21/92) were infected with Anaplasma sp. This Anaplasma sp. bacterium was close to a recently emerging Anaplasma platys strain based on gltA and groEL gene phylogenetic analysis. As to further understand the characteristics of Anaplasma sp., we raised a couple of positive goats (n = 2) in the laboratory with tick-free settings. We observed inappetence, vomiting, high fever, and weakness of limbs in the goat's offspring (n = 3). In addition, the blood samples from all offspring were all positive of this Anaplasma spp. We did not see any intracellular morulae in neutrophils, monocytes, and erythrocytes, but we identified some in the platelets of the blood smears from the positive goats by light microscopy. We named it A. platys-like and suggested it may infect platelets and be transmitted vertically through the placenta of goats. These findings deserve further evaluation.
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References
1.
Arraga-AlvaradoCM, QurolloBA, ParraOC, BerruetaMA, et al.Molecular evidence of Anaplasma platys infection in two women from Venezuela. Am J Trop Med Hyg, 2014; 91:1161–1165.
2.
Botelho-NeversE, SocolovschiC, RaoultD, ParolaP. Treatment of Rickettsia spp. infections: A review. Expert Rev Anti Infect Ther, 2012; 10:1425–1437.
3.
ChenSM, DumlerJS, BakkenJS, WalkerDH. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol, 1994; 32:589–595.
4.
ChochlakisD, IoannouI, SharifL, KokkiniS, et al.Prevalence of Anaplasma sp. in goats and sheep in Cyprus. Vector Borne Zoonotic Dis, 2009; 9:457–463.
5.
ChochlakisD, IoannouI, TselentisY, PsaroulakiA. Human anaplasmosis and Anaplasma ovis variant. Emerg Infect Dis, 2010; 16:1031–1032.
6.
CicuttinGL, TarragonaEL, De SalvoMN, MangoldAJ, et al.Infection with Ehrlichia canis and Anaplasma platys (Rickettsiales: Anaplasmataceae) in two lineages of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) from Argentina. Ticks Tick Borne Dis, 2015; 6:724–729.
7.
ČobádiováA, ReiterováK, DerdákováM, ŠpilovskáS, et al.Toxoplasma gondii, Neospora caninum and tick-transmitted bacterium Anaplasma phagocytophilum infections in one selected goat farm in Slovakia. Acta Parasitol, 2013; 58:541–546.
8.
DahmaniM, DavoustB, BenterkiMS, FenollarF, et al.Development of a new PCR-based assay to detect Anaplasmataceae and the first report of Anaplasma phagocytophilum and Anaplasma platys in cattle from Algeria. Comp Immunol Microbiol Infect Dis, 2015; 39:39–45.
9.
DumlerJS, BarbetAF, BekkerCP, DaschGA, et al.Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: Unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol, 2001; 51:2145–2165.
10.
Garcia-PerezAL, BarandikaJ, OportoB, PovedanoI, et al.Anaplasma phagocytophila as an Abortifacient Agent in Sheep Farms from Northern Spain. Ann N Y Acad Sci, 2003; 990:429–432.
11.
GeurdenT, SomersR, ThanhNT, VienLV, et al.Parasitic infections in dairy cattle around Hanoi, Northern Vietnam. Vet Parasitol, 2008; 153:384–388.
12.
GuoWP, HuangB, ZhaoQ, XuG, et al.Human-pathogenic Anaplasma spp., and Rickettsia spp. in animals in Xi'an, China. PLoS Negl Trop Dis, 2018; 12:e0006916.
13.
GuoWP, ZhangB, WangYH, XuG, et al.Molecular identification and Characteriza-tion of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China. BMC Infect Dis, 2019; 19:434.
14.
HarrusS, Perlman-AvrahamiA, MumcuogluKY, MorickD, et al.Molecular detection of Ehrlichia canis, Anaplasma bovis, Anaplasma platys, Candidatus Midichloria mitochondrii and Babesia canis vogeli in ticks from Israel. Clin Microbiol Infect, 2011; 17:459–463.
15.
HendrixGK, BraytonKA, BurchamGN. Anaplasma ovis as the suspected cause of mortality in a neonatal elk calf. J Vet Diagn Invest, 2019; 31:267–270.
16.
IsmailN, BlochKC, McBrideJW. Human ehrlichiosis and anaplasmosis. Clin Lab Med, 2010; 30:261–292.
17.
LiGH, YuQ, ZhangQX, PanL, et al.[Molecular epidemiology regarding Anaplasma phagocytophilum in Dermacentor silvarum in Ningwu county, Shanxi province]. Zhonghua Liu Xing Bing Xue Za Zhi, 2012; 33:642–643.
18.
LiH, ZhengYC, MaL, JiaN, et al.Human infection with a novel tick-borne Anaplasma species in China: A surveillance study. Lancet Infect Dis, 2015a; 15:663–670.
19.
LiY, YangJ, ChenZ, QinG, et al.Anaplasma infection of Bactrian camels (Camelus bactrianus) and ticks in Xinjiang, China. Parasit Vectors, 2015b; 8:313.
20.
LimaML, SoaresPT, RamosCA, AraújoFR, et al.Molecular detection of Anaplasma platys in a naturally-infected cat in Brazil. Braz J Microbiol, 2010; 41:381–385.
21.
LiuZ, MaM, WangZ, WangJ, et al.Molecular survey and genetic identification of Anaplasma species in goats from central and southern China. Appl Environ Microbiol, 2012; 78:464–470.
22.
LorussoV, WijnveldM, LatrofaMS, FajinmiA, et al.Canine and ovine tick-borne pathogens in camels, Nigeria. Vet Parasitol, 2016a; 228:90–92.
23.
LorussoV, WijnveldM, MajekodunmiAO, DongkumC, et al.Tick-borne pathogens of zoonotic and veterinary importance in Nigerian cattle. Parasit Vectors, 2016b; 9:217.
24.
LuM, TianJH, YuB, GuoWP, et al.Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. Ticks Tick Borne Dis, 2017; 8:574–580.
25.
MachadoRZ, TeixeiraMM, RodriguesAC, AndréMR, et al.Molecular diagnosis and genetic diversity of tick-borne Anaplasmataceae agents infecting the African buffalo Syncerus caffer from Marromeu Reserve in Mozambique. Parasit Vectors, 2016; 9:454.
26.
MathiesonAO. Ectoparasites and their control. In: MartinWB, AitkenID, eds. Diseases of Sheep, 2nd ed. Oxford: Blackwell Science Publication, 1991:284–289.
27.
PengY, WangK, ZhaoS, YanY, et al.Detection and phylogenetic characterization of: An emerging pathogen in sheep and goats in China. Front Cell Infect Microbiol, 2018; 8:283.
28.
PerronneC. Lyme and associated tick-borne diseases: Global challenges in the context of a public health threat. Front Cell Infect Microbiol, 2014; 4:74.
29.
RarV, GolovljovaI. Anaplasma, Ehrlichia, and “Candidatus Neoehrlichia” bacteria: Pathogenicity, biodiversity, and molecular genetic characteristics, a review. Infect Genet Evol, 2011; 11:1842–1861.
30.
ReppertE, GalindoRC, BreshearsMA, KocanKM, et al.Demonstration of transplacental transmission of a human isolate of Anaplasma phagocytophilum in an experimentally infected sheep. Transbound Emerg Dis, 2013; 60 (Suppl.): 93–96.
31.
RucksakenR, ManeeruttanarungrojC, MaswannaT, SussadeeM, et al.Comparison of conventional polymerase chain reaction and routine blood smear for the detection of Babesia canis, Hepatozoon canis, Ehrlichia canis, and Anaplasma platys in Buriram Province, Thailand. Vet World, 2019; 12:700–705.
32.
SilaghiC, SantosAS, GomesJ, ChristovaI, et al.Guidelines for the direct detection of Anaplasma spp. in diagnosis and epidemiological studies. Vector Borne Zoonotic Dis, 2017; 17:12–22.
33.
SimpsonRM, GauntSD, HairJA, KocanKM, et al.Evaluation of Rhipicephalus sanguineus as a potential biologic vector of Ehrlichia platys. Am J Vet Res, 1991; 52:1537–1541.
34.
StuenS. Anaplasma phagocytophilum—The most widespread tick-borne infection in animals in Europe. Vet Res Commun, 2007; 31(Suppl.):79–84.
35.
StuenS, GranquistEG, SilaghiC. Anaplasma phagocytophilum a widespread multi-host pathogen with highly adaptive strategies. Front Cell Infect Microbiol, 2013; 3:31.
36.
TamuraK, PetersonD, PetersonN, StecherG, et al.MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 2011; 28:2731–2739.
TayST, KohFX, KhoKL, OngBL. Molecular survey and sequence analysis of Anaplasma spp. in cattle and ticks in a Malaysian farm. Trop Biomed, 2014; 31:769–776.
39.
TorinaA, VicenteJ, AlongiA, ScimecaS, et al.Observed prevalence of tick-borne pathogens in domestic animals in Sicily, Italy during 2003–2005. Zoonoses Public Health, 2007; 54:8–15.
40.
WardropKJ, BirkenheuerA, BlaisMC, CallanMB, et al.Update on canine and feline blood donor screening for blood-borne pathogens. J Vet Intern Med, 2016; 30:15–35.
41.
YangJ, HanR, NiuQ, LiuZ, et al.Occurrence of four Anaplasma species with veterinary and public health significance in sheep, northwestern China. Ticks Tick Borne Dis, 2018; 9:82–85.
42.
YangJ, LiuZ, NiuQ, LiuJ, et al.A novel zoonotic Anaplasma species is prevalent in small ruminants: Potential public health implications. Parasit Vectors, 2017; 10:264.
43.
YasiniS, KhakiZ, RahbariS, KazemiB, et al.Hematologic and clinical aspects of experimental ovine anaplasmosis caused by Anaplasma ovis in Iran. Iran J Parasitol, 2012; 7:91–98.
44.
ZhangL, LiuH, XuB, ZhangZ, et al.Rural residents in China are at increased risk of exposure to tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. Biomed Res Int, 2014; 2014:313867.
45.
ZhangXC, ZhangLX, LiWH, WangSW, et al.Ehrlichiosis and zoonotic anaplasmo-sis in suburbanareas of Beijing, China. Vector Borne Zoonotic Dis, 2012; 12:932–937.
46.
ZhangY, LvY, ZhangF, ZhangW, et al.Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China. J Vet Sci, 2016; 17:523–529.
47.
ZobbaR, AnfossiAG, Pinna ParpagliaML, DoreGM, et al.Molecular investigation and phylogeny of Anaplasma spp. in Mediterranean ruminants reveal the presence of neutrophil-tropic strains closely related to A. platys. Appl Environ Microbiol, 2014; 80:271–280.
