Restricted accessResearch articleFirst published online 2011-12
Polymerase Chain Reaction Detection of Trypanosoma cruzi in Suboptimally Preserved Vectors and Comparative Infection Rates 2007–2010 in Escondido,Southern California
We investigate the effect of specimen preservation on reliable detection of infection rates of the Chagas disease agent Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) in the Californian endemic vector Triatoma protracta (Uhler) (Hemiptera: Reduviidae) by using polymerase chain reaction. Infection rates in suboptimally preserved specimens (dry or low-percentage ethanol) were found to be not significantly different from optimally preserved samples. Based on our samples, the infection rate of vectors in one population in Escondido, San Diego County, has not changed significantly between 2007 and 2010.
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References
1.
BernC, MontgomerySP, HerwaldtBL, RassiAJr.et al.Evaluation and treatment of Chagas disease in the United States: a systematic review. JAMA, 2007; 298:2171–2181.
2.
HwangW, ZhangG, MaslovD, WeirauchC. Infection rates of Triatoma protracta (Uhler) with Trypanosoma cruzi in Southern California and molecular identification of trypanosomes. Am J Trop Med Hyg, 2010; 83:1020–1022.
3.
KlotzSA, DornPL, KlotzJH, PinnasJLet al.Feeding behavior of triatomines from the southwestern United States: an update on potential risk for transmission of Chagas disease. Acta Trop, 2009; 111:114–118.
4.
NavinTR, RobertoRR, JuranekDD, LimpakarnjanaratKet al.Human and sylvatic Trypanosoma cruzi infection in California. Am J Public Health, 1985; 75:366–369.
5.
SoutoRP, FernandesO, MacedoAM, CampbellDAet al.DNA markers define two major phylogenetic lineages of Trypanosoma cruzi. Mol Biochem Parasitol, 1996; 83:141–152.
6.
WoodSF. Trypanosoma cruzi; new foci of enzootic Chagas disease in California. Exp Parasitol, 1975; 38:153–160.
